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Comparison of In Vitro Development of Mouse Early Blastocysts according to Vitrification and Conventional Slow Freezing Procedures

Oh DS, Lee KS

  • KMID: 2260975
  • Korean J Obstet Gynecol.
  • 1998 May;41(5):1470-1478.
This study was undertaken to investigate the rapid and optimum freezing procedure that can maintain a high survival rate after post-thaw blastocysts, determining the effects of vitrification and conventional slow...
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Comparative study on the development of frozen-thawed mouse embryo using vitrification at each developmental stage

Ahn KH, Oh JH, Lee JJ, Kim T, Kim SH

  • KMID: 2272438
  • Korean J Obstet Gynecol.
  • 2004 Nov;47(11):2159-2166.
OBJECTIVE: To find out the optimal freezing time in terms of developmental stage of mouse embryo and the effectiveness of vitrification method for freezing them. METHODS: Superovulation induction was performed using...
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Comparison of Vitrification and Slow Freezing-thawing Method on 1-cell Zygotes

Lee JH, Han HD, Koo HY

  • KMID: 1979129
  • Korean J Fertil Steril.
  • 2001 Sep;28(3):191-198.
OBJECTIVE: This study was conducted to examine the effect of vitrification on the survival and in vitro development of mice 1-cell zygotes. METHOD: Effects of exposure to vitrification solution and vitrification,...
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Comparison of Vitrification and Slow Freezing for the Cryopreservation of Mouse Pronuclear Stage Embryos

Kim MY, Lee YI

  • KMID: 2288278
  • Korean J Reprod Med.
  • 2007 Jun;34(2):117-124.
OBJECTIVE: The aim of this study was to compare the efficacy of slow freezing with vitrification method for cryopreservation of mouse pronuclear stage embryos. METHODS: Mouse pronuclear embryos obtained from superovulated...
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Vitrification and Ultrarapid Freezing of Day 2 Mouse Embryos

Yang JS, Sohn C, Bae IH

  • KMID: 2085144
  • Korean J Fertil Steril.
  • 2000 Sep;27(3):283-289.
OBJECTIVE: The study was performed to compare the survival rate and the development of day 2 mouse embryos which had freezing procedures done. METHODS: We used three different vitrification solutions (EFS,...
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Effect of Slush-nitrogen on the Cryopreservation of Oocytes and Embryos using Vitrification

Lee DR, Yoon TK

  • KMID: 1986909
  • Korean J Reprod Med.
  • 2009 Mar;36(1):1-7.
No abstract available.
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Evaluation of vitrification for cryopreservation of teeth

Dissanayake SC, Che ZM, Choi SH, Lee SJ, Kim J

PURPOSE: The aim of this study was to investigate whether vitrification in the cryopreservation of periodontal ligament (PDL) cells could be useful for tooth banking. METHODS: In step 1, primary cultured...
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Vitrification of Mouse Embryos in Ethylene Glycol-based Solutions

Kim MY, Lee ES, Lee SW, Lee YI

  • KMID: 2085176
  • Korean J Fertil Steril.
  • 2005 Jun;32(2):177-185.
OBJECTIVE: This study was conducted to find an optimal condition for the vitrification of mouse morulae and expanded blastocysts. MATERIALS AND METHODS: Mouse embryos were obtained at 2-cell stage and cultured...
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Rapid freezing versus Cryotop vitrification of mouse two-cell embryos

Inna N, Sanmee U, Saeng-anan U, Piromlertamorn W, Vutyavanich T

OBJECTIVE: To compare our in-house method of embryo freezing with Cryotop vitrification in terms of immediate survival, subsequent cleavage and blastocyst formation, and cell numbers in blastocysts. METHODS: Two-cell mouse embryos...
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Vitrification of Mouse Blastocyst

Lee YJ, Kim MY, Lee SW, Lee YI

  • KMID: 2077060
  • Korean J Obstet Gynecol.
  • 2004 Jul;47(7):1348-1354.
OBJECTIVE: The aim of this study was to compare the survival and developmental rate of two vitrification solutions for the vitrification of mouse expanded blastocysts. METHODS: Mouse embryos were obtained at...
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Cryopreservation of the Human Adult Ovarian Cortical Tissues by Vitrification

Lee KA, Lee SH, Ha SD, Yoon SJ, Ko JJ, Lee WS, Yoon TK, Cha KY

  • KMID: 2235737
  • Korean J Fertil Steril.
  • 1999 Aug;26(2):251-256.
The present study was conducted to evaluate whether vitrification could be used for ovarian tissue preservation. The important Issue here is that the vitrification is very simple, easy, and economical...
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Cryopreservation of Oocytes and Embryos by Vitrification

Gupta MK, Lee HT

  • KMID: 1458325
  • Korean J Reprod Med.
  • 2010 Dec;37(4):267-291.
Life can be kept in suspended animation either before fertilization at oocyte stage or after fertilization at different stages of embryonic development for a variety of reasons. It not only...
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Vitrification solution without sucrose for cryopreservation in mouse blastocysts

Joo JK, Lee YJ, Jeong JE, Kim SC, Ko GR, Lee KS

OBJECTIVE: This study was designed to investigate the survival rate of vitrified mouse blastocysts depending on the presence or absence of sucrose in vitrification solution. METHODS: Mouse two-cell embryos were collected...
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In vitro Follicular Growth and Ovulation of Mouse Preantral Follicles Cryopreserved by Vitrification

Park JK, Paik WY

  • KMID: 2085167
  • Korean J Fertil Steril.
  • 2005 Jun;32(2):91-99.
OBJECTIVE: To define an appropriate vitrification condition of preantral follicle that yields high survival and to evaluate growth and ovulation rate of mouse follicles during in vitro culture after vitrification. METHODS:...
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Effect of Cytochalasin B on Survivability and in Vitro Development of Mouse Oocyte Frozen by Vitrification

Choi WJ, Lee SA, Lee JH, Paik WY

  • KMID: 2271962
  • Korean J Obstet Gynecol.
  • 2003 Feb;46(2):317-322.
OBJECTIVE: The purpose of this study was to evaluate the effect of CCB treatment on the survivability and in vitro development of mouse oocyte frozen by vitrification. METHODS: Mouse oocytes retrieved...
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Closed vitrification of mouse oocytes using the CryoLogic vitrification method: A modification that improves developmental competence

Jo JW, Jee BC, Suh CS, Kim SH

OBJECTIVE: To compare the mouse oocyte vitrification outcomes of the CryoLogic vitrification method (CVM) and the conventional open method using a Cryotop. Two CVM methods (original CVM and modified CVM)...
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The Effect of Cytochalasin B on Cytoskeletal Stability of Mouse Oocyte Frozen by Vitrification

Paik WY, Choi WJ, Kim SN, Lee JH

  • KMID: 1566318
  • Korean J Fertil Steril.
  • 2002 Dec;29(4):229-236.
OBJECTIVE: The purpose of this study was to evaluate the effect of Cytochalasin B (CCB) on the cytoskeletal stability of mouse oocyte frozen by vitrification. METHODS: Mouse oocytes retrieved from cycle...
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Cryopreservation of Day 3 Mouse Embryos by Vitrification

Yoon SY, Sohn C, Bae IH

  • KMID: 2235765
  • Korean J Fertil Steril.
  • 1997 Dec;24(3):325-333.
The use of hormonal stimulation in human in vitro fertilization and embryo transfer (IVF-ET) leads to increased production of embryos for ET. So to avoid high pregnancies and to allow...
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Development of Effective Cryopreservation Method for Mouse Oocytes

Choi SJ, Kim SK, Kim JS, Cho JW, Jun JH, Byun HK

  • KMID: 1891203
  • Korean J Fertil Steril.
  • 2004 Mar;31(1):75-81.
OBJECTIVE: The purpose of this study was to evaluate the efficacy and effect of various cryopreservation method on the survival and the cytoskeletal stability of metaphase II mouse oocyte. METHODS: Mouse...
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Establishment of Mouse Oocytes Cryopreservation Program

Moon SY, Kim SH, Jee BC, Sung KC, Choi SM, Kim HS, Oh SK, Suh CS, Choi YM, Kim JG, Lee JY

  • KMID: 2262343
  • Korean J Obstet Gynecol.
  • 2001 Aug;44(8):1455-1463.
OBJECTIVE: To establish the optimal cryopreservation method in mouse oocytes. METHODS: Firstly, mouse immature oocytes were exposed to various cryoprotectants, and then cryoprotectant with the best outcome was selected. Secondly, mouse...
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