Korean J Fertil Steril.  2000 Sep;27(3):283-289.

Vitrification and Ultrarapid Freezing of Day 2 Mouse Embryos

• Yang JS
• Sohn C
• Bae IH

Abstract

OBJECTIVE
The study was performed to compare the survival rate and the development of day 2 mouse embryos which had freezing procedures done.
METHODS
We used three different vitrification solutions (EFS, VS14, DPS) and a ultrarapid freezing solution (UFS) for cryopreservation of day 2 mouse embryo.
RESULTS
We tested toxicity by exposing embryos to vitrification solutions and a ultrarapid freezing solution. The survival rates are 100%, 97.8%, 95.6% and 100% (EFS, VS14, DPS and UFS). After cultured for 96 hours, hatching rates of each group are 93.5% (no freezing), 95.6% (EFS), 86.4% (VS14), 93.0% (DPS), and 93.0% (UFS). There is no significant differences among groups. The survival rates after thawing cryopreserved embryos are 80.2%, 91.7%, 69.5%, 0% and 91.8% (slow freezing, EFS, VS14, DPS and UFS). Also cultured for 96 hours, the hatching rates are 93.5% (no freezing), 84.1% (slow freezing), 93.9%) (EFS), 48.5% (VS14) and 70.1% (UFS).
CONCLUSION
The survival rates of vitrification in EFS solution and ultrarapid freezing are higher than slow freezing (p<0.05). The hatching rate of vitrification in EFS solution cultured for 96 hours is highest, so vitrification of day 2 mouse embryos in EFS solution considered as more effective for cryopreservation.

MeSH Terms

Animals
Cryopreservation
Embryonic Structures*
Freezing*
Mice*
Survival Rate
Vitrification*