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Does conventional freezing affect sperm DNA fragmentation?

Le MT, Nguyen TT, Nguyen TT, Nguyen TV, Nguyen TA, Nguyen QH, Cao TN

OBJECTIVE: Sperm cryopreservation has been widely used in assisted reproductive technology, as it offers great potential for the treatment of some types of male infertility. However, cryopreservation may result in...
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Rapid freezing versus Cryotop vitrification of mouse two-cell embryos

Inna N, Sanmee U, Saeng-anan U, Piromlertamorn W, Vutyavanich T

OBJECTIVE: To compare our in-house method of embryo freezing with Cryotop vitrification in terms of immediate survival, subsequent cleavage and blastocyst formation, and cell numbers in blastocysts. METHODS: Two-cell mouse embryos...
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Stimulation in Supplementary Motor Area Versus Motor Cortex for Freezing of Gait in Parkinson's Disease

Kim SJ, Paeng SH, Kang SY

BACKGROUND AND PURPOSE: Freezing of gait (FOG) is a frustrating problem in Parkinson's disease (PD) for which there is no effective treatment. Our aim was to find brain stimulation areas...
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Freezing and Washing of Red Blood Cells Using Haemonetics ACP 215

Youn KW, Choi KY, Lee SA, Min HK, Kim J

BACKGROUND: The use of a functionally closed system for the glycerolization and deglycerolization of red blood cells (RBCs) allows for prolonged post-thaw storage for more than 24 hours. The aim...
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Tube Erosion with Scleral Melting after Ahmed Valve Implantation Using a Synthetic Dural Substitute

Ahn HM, Park JW

PURPOSE: The objective of this case report was to present tube erosion of Ahmed valve implantation using a synthetic dura substitute (Neuro-Patch®, B. Braun, Boulogne, France). CASE SUMMARY: Tube erosion was...
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Comparison of the bond strength of ceramics to Co-Cr alloys made by casting and selective laser melting

Lawaf S, Nasermostofi S, Afradeh M, Azizi A

PURPOSE: Considering the importance of metal-ceramic bond, the present study aimed to compare the bond strength of ceramics to cobalt-chrome (Co-Cr) alloys made by casting and selective laser melting (SLM). MATERIALS...
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Effect of Drug Carrier Melting Points on Drug Release of Dexamethasone-Loaded Microspheres

Park JH, Kwon DY, Heo JY, Park SH, Park JY, Lee B, Kim JH, Kim MS

Here, we examined the effect of melting point of drug carriers on drug release of dexamethasone (Dex)-loaded microspheres. We prepared poly(L-lactide-ran-ε-caprolactone) (PLC) copolymers with varying compositions of poly(εcaprolactone) (PCL) and...
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Acceptance of Oocytes Freezing for Fertility Preservation for Social Reasons Among Unmarried Korean Women

Cho HS, Kim HO

PURPOSE: To evaluate the awareness of unmarried Korean women about the relationship between fertility and age with respect to the need to consider oocyte freezing to preserve fertility in cases...
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Freezing of Gait in Extrapontine Myelinolysis

Song J, Kwon YN, Na BS, Song SJ, Shin YY, Yi JH, Lee D, Ahn TB

A 65-year-old female visited us due to gait disturbance. A neurological examination showed cognitive impairment, dystonia, myoclonus, bradykinesia, postural instability, and freezing of gait (FOG). She was diagnosed with extrapontine...
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A Forensic Entomology Case Estimating the Minimum Postmortem Interval Using the Distribution of Fly Pupae in Fallow Ground and Maggots with Freezing Injury

Shin SE, Jang MS, Park JH, Park SH

Forensic entomology investigates postmortem interval (PMI) estimation using insect evidence. We estimated the minimum PMI of a putrefied male cadaver using pupae in the soil and maggots found in the...
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Cryopreservation of Human Wharton's Jelly-derived Mesenchymal Stem Cells Following Controlled Rate Freezing Protocol Using Different Cryoprotectants; A Comparative Study

Shivakumar SB, Bharti D, Jang SJ, Hwang SC, Park JK, Shin JK, Byun JH, Park BW, Rho GJ

OBJECTIVES: To compare the effect of three different cryoprotectants on basic stem cell characteristics for the possibility of using well defined, dimethyl sulfoxide (DMSO) and serum free freezing solutions to...
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High Frequency Repetitive Transcranial Magnetic Stimulation for Freezing of Gait and Nonmotor Symptoms in Parkinson's Disease

Oh E, Park S, Lim J, Lee AY, Bok SK, Song HJ

BACKGROUND: To investigate the effect of high frequency repetitive transcranial magnetic stimulation (rTMS) on motor symptoms especially freezing of gait (FoG), and nonmotor symptoms in Parkinson disease (PD). METHODS: In this...
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Stability and Sterility of Bevacizumab after Withdrawal into a Syringe and Refrigeration or Freezing

Noh GM, Kim SY, Nam KY, Lee SU, Kim YH, Lee SJ

PURPOSE: The aim of this study was to evaluate the stability and sterility of bevacizumab (Avastin, Genentech, Inc., San Francisco, CA, USA) after withdrawal into multiple doses from single-use vials. METHODS:...
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Comparison of Vitrification and Slow Freezing for the Cryopreservation of Mouse Pronuclear Stage Embryos

Kim MY, Lee YI

  • KMID: 2288278
  • Korean J Reprod Med.
  • 2007 Jun;34(2):117-124.
OBJECTIVE: The aim of this study was to compare the efficacy of slow freezing with vitrification method for cryopreservation of mouse pronuclear stage embryos. METHODS: Mouse pronuclear embryos obtained from superovulated...
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The effectiveness of ethylene glycol as cryoprotectant in mouse embryo freezing with slow freezing method

Kim YJ, Kim OK, Park EA, Hong SC, Yu SY, Kim T, Lee JJ, Oh JH, Kim SH

  • KMID: 2272847
  • Korean J Obstet Gynecol.
  • 2006 Jul;49(7):1481-1491.
OBJECTIVE: We intended to know how the cryoprotectant ethylene glycol (EG) would affect the outcome of the embryo development when used in slow freezing method. And to know if there...
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Ideal Freezing Curve Can Avoid the Damage by Latent Heat of Fusion During Freezing

Park HK, Park YH, Yoon WS, Kim TS, Yoon CS, Kim SH, Lim SH, Kim JH, Kwak YT, Han DW, Park JC, Cho BK

  • KMID: 2062669
  • Korean J Thorac Cardiovasc Surg.
  • 2003 Apr;36(4):219-228.
BACKGROUND: Liquid nitrogen freezing techniques have already met with widespread success in biology and medicine as a means of long-term storage for cells and tissues. The use of cryoprotectants such...
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Freezing and Thawing Conditions of Rat Hepatocytes

Hwang SG, Jung EM, Oh SW, Kwon CI, Kim KC, Hong SP, Park PW, Rim KS

  • KMID: 1890007
  • Korean J Hepatol.
  • 2001 Sep;7(3):308-314.
BACKGROUND/AIMS: During cryopreservation of hepatocytes, a dramatic loss in cell number, viability and differentiated cell function is usually inevitable because hepatocytes are very sensitive to stress during freezing and thawing....
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Freezing Therapy for Relief of Vasospasm on Microvascular Anastomosis with Damaged Endothelium: An Experimental and Clinical Application

Kim YK, Park HS, Yoon CH

  • KMID: 1942500
  • J Korean Soc Plast Reconstr Surg.
  • 2001 Jul;28(4):391-396.
The histologically confirmed endothelial damage, which appears as a normal vessel under the operative microscope, may lead to thrombus formation or vasospasm, and ultimately result in the anastomotic failure. Vascular...
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Rapid freezing of umbilical cord blood in a -80 degrees C freezer

Lee JA, Yoo KH, Kim DH, Sung KW, Jung HL, Koo HH, Park Q, Kim DW

  • KMID: 1862972
  • Korean J Blood Transfus.
  • 2001 Jun;12(1):55-62.
BACKGROUND: As the number of umbilical cord blood (UCB) transplants are increasing, well standardized processing of UCB units with high quality is needed. The aim of the study was to...
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A Scanning Electron Microscopic Study about Crush Injured Vasospasm after Freezing Therapy

Kang SR, Kim YK, Park HS, Yoon CH

  • KMID: 2322479
  • J Korean Soc Plast Reconstr Surg.
  • 2001 May;28(3):262-267.
The histologically confirmed endothelial damage (HCED) may lead to vasospasm, which increases the failure rate of microvascular anastomosis. Recently, vascular freezing has been reported to be the most effective and...
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