Yonsei Med J.  1995 Aug;36(4):332-335. 10.3349/ymj.1995.36.4.332.

The biomechanical properties of deep freezing and freeze drying bones and their biomechanical changes after in-vivo allograft

• Kang JS
• Kim NH

• KMID: 1066883
• DOI: http://doi.org/10.3349/ymj.1995.36.4.332

Abstract

This study measured the physical properties in bending of the rat femur and compression of the rat first tail vertebra subjected to deep freezing at -80 degrees C for 2 weeks, 6 weeks, 12 weeks and freeze drying. This study also measured the mechanical changes after in vivo allograft of fresh bone, deep freezing(2, 6, 12 weeks) and freeze drying. Analysis for deep freezing groups showed a mean 7.2% decrease in bending strength and 11.0% decrease in compressive strength when compared with the control group, but there was no statistical difference in the duration of deep freezing. The groups of in vivo graft after deep freezing showed 23.1% and 22.2% decrease in bending and compressive strength. There was no statistical difference in the duration of deep freezing. The freeze drying group showed a 9.7% decrease in bending strength and no significant difference in compressive strength. The group of in vivo graft after freeze drying showed a 30.1% and a 41.3% decrease in bending and compressive strength. The above results suggested that there would be some mechanical limitation in using freeze dried graft for supporting implants.

Keyword

Bone; allograft; freezing; freeze drying; biomechanics

MeSH Terms

Animal
Biomechanics
*Bone Transplantation
Bone and Bones/*physiopathology
Femur/physiopathology
*Freeze Drying
*Freezing
Postoperative Period
Rats
Rats, Sprague-Dawley
Spine/physiopathology
Tail
Transplantation, Homologous