Tuberc Respir Dis.  1974 Sep;21(3):144-148. 10.4046/trd.1974.21.3.144.

Experimental study on production of Freeze Dried B.C.G. Vaccine in Korea

• KMID: 2529011
• DOI: http://doi.org/10.4046/trd.1974.21.3.144

Abstract

Author Prepared 30 lots of Freeze dried B.C.G. Vaccine with using Stokes Freeze dryer. The vaccine was dried for 24 hours. in a vacuum of 200 micron at 25 ℃, after few hours initiate freezing at -40 ℃, The number of viable count was determined by the quantitative culture on Löwenstein. Jensen medium. The survival rate was 37% compared before freeze drying and the viavle rate after 3 months. storage at 5℃ was 81 % and 56% after 6 months. ln regard to the tuberculin conversion rate to schoolchildren, the freeze dried B.C.G. vaccine showed 66% comparing with the wet B.C.G. vaccine which showed 76%. And the size of average induration by the freeze dried vaccine was a little smaller than that by the' wet vaccine, the size of the vaccination lesion by the freeze dried B.C.G. vaccine was smaller then that of wet B.C.G. vaccine.