Korean J Physiol Pharmacol.  2000 Aug;4(4):283-289.

Effect of atrial natriuretic peptide on the proliferation and activity of osteoblastic cells

• Lee JR
• Ko SY
• Kim JK
• Kim SW

Abstract

Natriuretic peptides comprise a family of three structurally related peptides; atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). The present study was performed to investigate the effect of ANP on the proliferation and activity of ROS17/2.8 and HOS cells which are well-characterized osteoblastic cell lines. ANP dose-dependently decreased the number of ROS17/2.8 and HOS cells after 48-hour treatment. ANP generally increased the alkaline phosphatase activity of ROS17/2.8 and HOS cells after 48 hr treatment, regardless of the fact that basal activity of alkaline phosphatase was much lower in HOS cells compared to that of ROS17/1.8 cells. ANP increased the NBT reduction by ROS17/2.8 and HOS cells. ANP showed the variable but no significant effect on the nitric oxide production by ROS17/2.8 and HOS cells. ROS17/2.8 and HOS cells produced and secreted gelatinase into culture medium, and this enzyme was thought to be the gelatinase A type with the molecular weight determination. The gelatinase activity produced by ROS17/2.8 cells was increased by the treatment of ANP. However, the enzyme activity was not affected by ANP treatment in the HOS cell culture. In summary, ANP decreased the proliferation and increased the alkaline phosphatase activity and NBT reduction of osteoblasts. These results indicate that ANP is one of the important regulators of bone metabolism.

MeSH Terms

Alkaline Phosphatase
Atrial Natriuretic Factor
Cell Culture Techniques
Cell Line
Gelatinases
Humans
Matrix Metalloproteinase 2
Metabolism
Molecular Weight
Natriuretic Peptide, Brain
Natriuretic Peptide, C-Type
Natriuretic Peptides
Nitric Oxide
Osteoblasts*
Peptides
Alkaline Phosphatase
Atrial Natriuretic Factor
Gelatinases
Matrix Metalloproteinase 2
Natriuretic Peptide, Brain
Natriuretic Peptide, C-Type
Natriuretic Peptides
Nitric Oxide
Peptides