J Korean Soc Endocrinol.  1998 Mar;13(1):34-44.

The Effect of Sex Steroid Hormone on the Expression of Insulin-Like Growth Factor Binding Preteins mRNA in the Explant Cultured from Human Uterine Myoma and Adjacent Normal Myometrium

• Lee JY
• Kim JK
• Seo CS
• Kim SH
• Choi YM
• Moon SY

Abstract

BACKGROUND: Sex steroid hormones are believed to play an important role in the genesis and growth of uterine myoma. Several studies suggest a possible role of insulin-like growth factor(IGF) as a mediator of estradiol in uterine myama. We have recently demonstrated that some IGF binding proteins(IGFRPs) messenger ribonucleic acid(mRNA) expressions in myoma are dependent on the in vivo esttogen status. The purposes of this study are to evaluate the in vitro effects of sex steroid hormones including estrogen on the IGFBPs gene expression in tissues from uterine myoma and adjacent normal myometrium.
METHODS
Tissues from myoma and adjacent normal myometrium of patients with uterine myoma during early proliferative phase of menstrual cycle were cultured in the absence(control) and presence of 17b-estradiol(10M/L) or/and progesterone(10M/L) for 3 days. The IGFBPs mRNA expressions in these explants were analyzed by Nothern blot using specific human complementary deoxyribonucleic acid(cDNA) probes.
RESULTS
The addition of 17b-estradiol, progesterone alone and in combination to conditioned media of explants from myoma and adjacent normal myornetrium did not result in any changes in the expression of IGFBP-2, IGFBP-4, IGFBP-5, and IGFBP-6 mRNA. With progesterone addtion, lGFBP-3 rnRNA expression was significantly reduced in myoma explant but not in adjacent ncemal myometrium explant. There was no significant change in the IGFBP-3 mRNA expression with 17b-estradiol and with the combination of both 17b-estradiol and progesterone.
CONCLUSION
17b-estradiol does not affect IGFBPs gene expression in the myoma and adjacent normal myometrium explant regardless of the presence of progesterone in vitro. However progesterone alone induces a decrease in IGFBP-3 synthesis in myoma explant.

MeSH Terms

Animals
Culture Media, Conditioned
Estradiol
Estrogens
Female
Gene Expression
Gonadal Steroid Hormones
Humans*
Insulin-Like Growth Factor Binding Protein 2
Insulin-Like Growth Factor Binding Protein 3
Insulin-Like Growth Factor Binding Protein 4
Insulin-Like Growth Factor Binding Protein 5
Insulin-Like Growth Factor Binding Protein 6
Insulin-Like Growth Factor Binding Proteins
Leiomyoma*
Menstrual Cycle
Mice
Myoma
Myometrium*
Progesterone
RNA, Messenger*
Culture Media, Conditioned
Estradiol
Estrogens
Gonadal Steroid Hormones
Insulin-Like Growth Factor Binding Protein 2
Insulin-Like Growth Factor Binding Protein 3
Insulin-Like Growth Factor Binding Protein 4
Insulin-Like Growth Factor Binding Protein 5
Insulin-Like Growth Factor Binding Protein 6
Insulin-Like Growth Factor Binding Proteins
Progesterone
RNA, Messenger