Korean J Parasitol.
2014 Jun;52(3):251-256.
A Novel Recombinant BCG Vaccine Encoding Eimeria tenella Rhomboid and Chicken IL-2 Induces Protective Immunity Against Coccidiosis
- Affiliations
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- 1Hebei Normal University of Science and Technology, Key Laboratory of Preventive Veterinary Medicine of Hebei Province, Qinhuangdao 066600, China.
- 2College of Animal Science and Veterinary Medicine, JiLin University, Changchun 130062, China. xczhang@jlu.edu.cn
- 3Hebei Normal University of Science and Technology, Foreign language institute, Qinhuangdao 066600, China.
Abstract
- A novel recombinant Bacille Calmette-Guerin (rBCG) vaccine co-expressed Eimeria tenella rhomboid and cytokine chicken IL-2 (chIL-2) was constructed, and its efficacy against E. tenella challenge was observed. The rhomboid gene of E. tenella and chIL-2 gene were subcloned into integrative expression vector pMV361, producing vaccines rBCG pMV361-rho and pMV361-rho-IL2. Animal experiment via intranasal and subcutaneous route in chickens was carried out to evaluate the immune efficacy of the vaccines. The results indicated that these rBCG vaccines could obviously alleviate cacal lesions and oocyst output. Intranasal immunization with pMV361-rho and pMV361-rho-IL2 elicited better protective immunity against E. tenella than subcutaneous immunization. Splenocytes from chickens immunized with either rBCG pMV361-rho and pMV361-rho-IL2 had increased CD4+ and CD8+ cell production. Our data indicate recombinant BCG is able to impart partial protection against E. tenella challenge and co-expression of cytokine with antigen was an effective strategy to improve vaccine immunity.
Keyword
MeSH Terms
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Adjuvants, Immunologic/genetics/*metabolism
Administration, Intranasal
Animals
Antigens, Protozoan/genetics/*immunology
BCG Vaccine/administration & dosage/*genetics
CD4-Positive T-Lymphocytes/immunology
CD8-Positive T-Lymphocytes/immunology
Chickens
Coccidiosis/*prevention & control
Disease Models, Animal
Drug Carriers/administration & dosage
Eimeria tenella/genetics/*immunology
Genetic Vectors
Injections, Subcutaneous
Interleukin-2/genetics/*metabolism
Protozoan Vaccines/administration & dosage/genetics/*immunology
Spleen/immunology
Vaccines, Synthetic/administration & dosage/genetics/immunology
Adjuvants, Immunologic
Antigens, Protozoan
BCG Vaccine
Drug Carriers
Interleukin-2
Protozoan Vaccines
Vaccines, Synthetic
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