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Evaluation of Recombinant SAG1, SAG2, and SAG3 Antigens for Serodiagnosis of Toxoplasmosis

Khanaliha , Motazedian MH, Kazemi B, Shahriari , Bandehpour M, Sharifniya Z

  • KMID: 1792160
  • Korean J Parasitol.
  • 2014 Apr;52(2):137-142.
Serologic tests are widely accepted for diagnosing Toxoplasma gondii but purification and standardization of antigen needs to be improved. Recently, surface tachyzoite and bradyzoite antigens have become more attractive for...
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New Molecules in Babesia gibsoni and Their Application for Diagnosis, Vaccine Development, and Drug Discovery

Goo YK, Xuan X

Babesia gibsoni is an intraerythrocytic apicomplexan parasite that causes piroplasmosis in dogs. B. gibsoni infection is characterized clinically by fever, regenerative anemia, splenomegaly, and sometimes death. Since no vaccine is...
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Genetic Polymorphisms in VIR Genes among Indian Plasmodium vivax Populations

Gupta P, Pande V, Das A, Singh V

The vir genes are antigenic genes and are considered to be possible vaccine targets. Since India is highly endemic to Plasmodium vivax, we sequenced 5 different vir genes and investigated...
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Evolution of Genetic Polymorphisms of Plasmodium falciparum Merozoite Surface Protein (PfMSP) in Thailand

Kuesap J, Chaijaroenkul W, Ketprathum K, Tattiyapong P, Na-Bangchang K

Plasmodium falciparum malaria is a major public health problem in Thailand due to the emergence of multidrug resistance. The understanding of genetic diversity of malaria parasites is essential for developing...
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Detection of Toxoplasma antigens and antibodies in mice infected with different strains of Toxoplasma gondii

Lee YH, Kim KY, Kang MS, Shin DW

This study aims to assess the possible strain-dependent variations in detection of Toxoplasma antigens and antibodies. The virulent RH strain or avirulent Beverley strain of T. gondii were injected into...
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Antibody reaction of human anti-Toxoplasma gondii positive and negative sera with Neospora caninum antigens

Nam HW, Kang SW, Choi WY

Anti-Neospora caninum antibody was detected in anti-Toxoplasma gondii positive and negative human sera by ELISA, western blot and immunofluorescence assay (IFA). Twelve cases out of 172 (6.7%) Toxoplasma-positive sera...
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Rapid detection of Theileria sergenti by polymerase chain reaction

Choi EJ, Kang SW, Kweon CH, Jeong WS, Yoon YD, Song HJ

Four separate pairs of oligonucleotide primers within the coding region in a T. sergenti 33-kDa surface protein gene were selected to detect T. sergenti by PCR. The specificity of PCR-amplified...
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Antibody Responses to Cryptosporidium Antigen in HIV-positive Patients in the Republic of Korea

Guk SM, Chai JY, Shin YO, Seo M

The diagnosis of cryptosporidiosis has been carried out using coprologic techniques in the Republic of Korea. However, antibody responses to Cryptosporidium have rarely been studied. Serum antibodies from HIV-positive/oocyst-positive Korean...
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Breast-Feeding Protects Infantile Diarrhea Caused by Intestinal Protozoan Infections

Abdel-Hafeez EH, Belal US, Abdellatif MZM, Naoi K, Norose K

  • KMID: 1792142
  • Korean J Parasitol.
  • 2013 Oct;51(5):519-524.
This study investigated the effect of breast-feeding in protection against protozoan infection in infants with persistent diarrhea. Infants were classified into 2 groups; 161 breast-fed infants and the same number...
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Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against experimental Neospora caninum infection

Cho JH, Chung WS, Song KJ, Na BK, Kang SW, Song CY, Kim TS

Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against N. caninum infection was evaluated in vitro and in vivo. Two major immunodominant surface antigens (NcSAG1 and NcSRS2) and...
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Genetic Characteristics of Polymorphic Antigenic Markers among Korean Isolates of Plasmodium vivax

Hwang SY, Kim SH, Kho WG

Plasmodium vivax, a protozoan malaria parasite of humans, represents a major public health concern in the Republic of Korea (= South Korea). However, little is known about the genetic properties...
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Diagnostic Usefulness of Malaria Antigen and Antibody Rapid Diagnostic Tests for Differential Diagnosis of Plasmodium Vivax Malaria in a Local Emergency Medical Center

Baek SH, Shin DW, Park JS, Roh JY, Kim H, Park JM, Kim KH, Kim HJ

  • KMID: 2332851
  • J Korean Soc Emerg Med.
  • 2010 Dec;21(6):872-877.
PURPOSE: A positive finding on a peripheral blood smear is the gold standard for the diagnosis of malaria. However, even for an expert microscopist, this test is a laborious and...
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Identification of novel Leishmania major antigens that elicit IgG2a response in resistant and susceptible mice

Mohammadi , Zeinali M, Ardestani , Kariminia

Experimental murine models with high, intermediate and low levels of genetically based susceptibility to Leishmania major infection reproduce almost entire spectrum of clinical manifestations of the human disease. There are...
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Efficacy of a DNA Vaccine Carrying Eimeria maxima Gam56 Antigen Gene against Coccidiosis in Chickens

Xu J, Zhang Y, Tao J

To control coccidiosis without using prophylactic medications, a DNA vaccine targeting the gametophyte antigen Gam56 from Eimeria maxima in chickens was constructed, and the immunogenicity and protective effects were evaluated....
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High Expression of Water-Soluble Recombinant Antigenic Domains of Toxoplasma gondii Secretory Organelles

Yang Z, Ahn HJ, Nam HW

Recombinant antigenic proteins of Toxoplasma gondii are alternative source of antigens which are easily obtainable for serodiagnosis of toxoplasmosis. In this study, highly antigenic secretory organellar proteins, dense granular GRA2...
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Antigenemia and Specific IgM and IgG Antibody Responses in Rabbits Infected with Toxoplasma gondii

Quan JH, Hassan HA, Cha GH, Shin DW, Lee YH

In this experiment, the correlation between antigenemia and specific antibody responses in Toxoplasma gondii-infected rabbits was assessed. We injected 1,000 T. gondii tachyzoites (RH) subcutaneously into 5 rabbits. Parasitemia, circulating...
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Primary structure of mature SAG1 gene of an Indonesian Toxoplasma gondii and comparison with other strains

Hartati S, Kusumawati A, Wuryastuti H, Widada JS

Toxoplasma gondii is a persistent protozoan parasite capable of infecting almost any warm-blooded vertebrates. SAG1 (p30) is the prototypic member of a superfamily of surface antigens called SRS (SAG1-related sequence)....
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Allelic Diversity and Geographical Distribution of the Gene Encoding Plasmodium falciparum Merozoite Surface Protein-3 in Thailand

Sawaswong , Simpalipan P, Siripoon N, Harnyuttanakorn P, Pattaradilokrat S

Merozoite surface proteins (MSPs) of malaria parasites play critical roles during the erythrocyte invasion and so are potential candidates for malaria vaccine development. However, because MSPs are often under strong...
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Serological observation of Toxoplasma gondii prevalence in Apodemus agrarius, a dominant species of field rodents in Korea

Jeon SH, Yong TS

Field rodents involved in ecological food chains and which are the prey of carnivores in the natural environment may serve as reservoir hosts for Toxoplasma gondii infection in humans, however,...
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Five cases of ocular toxocariasis confirmed by serology

Park SP, Park I, Park HY, Lee SU, Huh S, Magnaval J

We report 5 cases of ocular toxocariasis in Korean adults complaining of visual impairment along with floating or bubbling sensation. Fundoscopic examination revealed a retinal detachment along with exudate...
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