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Allelic Diversity and Geographical Distribution of the Gene Encoding Plasmodium falciparum Merozoite Surface Protein-3 in Thailand

Sawaswong , Simpalipan P, Siripoon N, Harnyuttanakorn P, Pattaradilokrat S

Merozoite surface proteins (MSPs) of malaria parasites play critical roles during the erythrocyte invasion and so are potential candidates for malaria vaccine development. However, because MSPs are often under strong...
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Plasmodium falciparum Genotype Diversity in Artemisinin Derivatives Treatment Failure Patients along the Thai-Myanmar Border

Congpuong , Hoonchaiyapoom T, Inorn K

Genetic characteristics of Plasmodium falciparum may play a role in the treatment outcome of malaria infection. We have studied the association between diversity at the merozoite surface protein-1 (msp-1), msp-2,...
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Prominent IL-12 Production and Tumor Reduction in Athymic Nude Mice after Toxoplasma gondii Lysate Antigen Treatment

Pyo KH, Jung BK, Xin CF, Lee YW, Chai JY, Shin EH

Toxoplasma gondii is an intracellular protozoan parasite that causes a Th1 cellular immunity. Our previous study showed that T. gondii lysate antigen (TLA) treatment in S180 tumor-bearing mice resulted in...
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Genetic Polymorphisms in VIR Genes among Indian Plasmodium vivax Populations

Gupta P, Pande V, Das A, Singh V

The vir genes are antigenic genes and are considered to be possible vaccine targets. Since India is highly endemic to Plasmodium vivax, we sequenced 5 different vir genes and investigated...
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Evaluation of the Accuracy of the EasyTest(TM) Malaria Pf/Pan Ag, a Rapid Diagnostic Test, in Uganda

Chong CK, Cho PY, Na BK, Ahn SK, Kim JS, Lee JS, Lee SK, Han ET, Kim HY, Park YK, Cha SH, Kim TS

In recent years, rapid diagnostic tests (RDTs) have been widely used for malaria detection, primarily because of their simple operation, fast results, and straightforward interpretation. The Asan EasyTest(TM) Malaria Pf/Pan...
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High Expression of Water-Soluble Recombinant Antigenic Domains of Toxoplasma gondii Secretory Organelles

Yang Z, Ahn HJ, Nam HW

Recombinant antigenic proteins of Toxoplasma gondii are alternative source of antigens which are easily obtainable for serodiagnosis of toxoplasmosis. In this study, highly antigenic secretory organellar proteins, dense granular GRA2...
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New Molecules in Babesia gibsoni and Their Application for Diagnosis, Vaccine Development, and Drug Discovery

Goo YK, Xuan X

Babesia gibsoni is an intraerythrocytic apicomplexan parasite that causes piroplasmosis in dogs. B. gibsoni infection is characterized clinically by fever, regenerative anemia, splenomegaly, and sometimes death. Since no vaccine is...
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A Novel Recombinant BCG Vaccine Encoding Eimeria tenella Rhomboid and Chicken IL-2 Induces Protective Immunity Against Coccidiosis

Wang Q, Chen L, Li J, Zheng J, Cai N, Gong P, Li S, Li H, Zhang X

  • KMID: 1792178
  • Korean J Parasitol.
  • 2014 Jun;52(3):251-256.
A novel recombinant Bacille Calmette-Guerin (rBCG) vaccine co-expressed Eimeria tenella rhomboid and cytokine chicken IL-2 (chIL-2) was constructed, and its efficacy against E. tenella challenge was observed. The rhomboid gene...
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Probability of Antibody Formation against Circumsporozoite Protein of Plasmodium vivax among Korean Malaria Patients

Nam HW, Song KJ, Ahn HJ, Yang Z, Chong CK, Cho PY, Ahn SK, Kim TS

  • KMID: 1792161
  • Korean J Parasitol.
  • 2014 Apr;52(2):143-149.
To evaluate the seroprevalence against circumsporozoite protein (CSP) of Plasmodium vivax in sera of Korean patients, the central repeating domain (CRD) of CSP was cloned and analyzed. From the genomic...
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Evaluation of Recombinant SAG1, SAG2, and SAG3 Antigens for Serodiagnosis of Toxoplasmosis

Khanaliha , Motazedian MH, Kazemi B, Shahriari , Bandehpour M, Sharifniya Z

  • KMID: 1792160
  • Korean J Parasitol.
  • 2014 Apr;52(2):137-142.
Serologic tests are widely accepted for diagnosing Toxoplasma gondii but purification and standardization of antigen needs to be improved. Recently, surface tachyzoite and bradyzoite antigens have become more attractive for...
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Evolution of Genetic Polymorphisms of Plasmodium falciparum Merozoite Surface Protein (PfMSP) in Thailand

Kuesap J, Chaijaroenkul W, Ketprathum K, Tattiyapong P, Na-Bangchang K

Plasmodium falciparum malaria is a major public health problem in Thailand due to the emergence of multidrug resistance. The understanding of genetic diversity of malaria parasites is essential for developing...
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Breast-Feeding Protects Infantile Diarrhea Caused by Intestinal Protozoan Infections

Abdel-Hafeez EH, Belal US, Abdellatif MZM, Naoi K, Norose K

  • KMID: 1792142
  • Korean J Parasitol.
  • 2013 Oct;51(5):519-524.
This study investigated the effect of breast-feeding in protection against protozoan infection in infants with persistent diarrhea. Infants were classified into 2 groups; 161 breast-fed infants and the same number...
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A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein

Song KJ, Yang Z, Chong CK, Kim JS, Lee KC, Kim TS, Nam HW

  • KMID: 1792140
  • Korean J Parasitol.
  • 2013 Oct;51(5):503-510.
Toxoplasma gondii is an apicomplexan parasite with a broad host range of most warm-blooded mammals including humans, of which one-thirds of the human population has been infected worldwide which can...
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Efficacy of a DNA Vaccine Carrying Eimeria maxima Gam56 Antigen Gene against Coccidiosis in Chickens

Xu J, Zhang Y, Tao J

To control coccidiosis without using prophylactic medications, a DNA vaccine targeting the gametophyte antigen Gam56 from Eimeria maxima in chickens was constructed, and the immunogenicity and protective effects were evaluated....
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Kinetics of IL-23 and IL-12 Secretion in Response to Toxoplasma gondii Antigens from THP-1 Monocytic Cells

Quan JH, Zhou W, Cha GH, Choi IW, Shin DW, Lee YH

IL-23 and IL-12 are structurally similar and critical for the generation of efficient cellular immune responses. Toxoplasma gondii induces a strong cell-mediated immune response. However, little is known about IL-23...
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Production and Evaluation of Toxoplasma gondii Recombinant GRA7 for Serodiagnosis of Human Infections

Selseleh M, Keshavarz , Mohebali M, Shojaee S, Selseleh M, Eshragian , Mansouri F, Modarressi MH

The precise diagnosis of the acute toxoplasmosis in pregnant women and immunocompromsied patients has critical importance. Most of the commercially available assays use the whole Toxoplasma soluble extract as the...
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Seroprevalence of Toxoplasmosis in the Residents of Cheorwon-gun, Gangwon-do, Korea

Ahn HJ, Cho PY, Ahn SK, Kim TS, Chong CK, Hong SJ, Cha SH, Nam HW

The seroepidemiological status of toxoplasmosis was surveyed among the residents of Cheorwon-gun, Gangwon-do by means of ELISA using a crude extract antigen of Toxoplasma gondii. The sera of 1,661 adult...
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Real-Time RT-PCR on SAG1 and BAG1 Gene Expression during Stage Conversion in Immunosuppressed Mice Infected with Toxoplasma gondii Tehran Strain

Selseleh M, Modarressi MH, Mohebali M, Shojaee S, Eshragian , Selseleh M, Azizi E, Keshavarz

Toxoplasmic encephalitis is caused by reactivation of bradyzoites to rapidly dividing tachyzoites of the apicomplexan parasite Toxoplasma gondii in immunocompromised hosts. Diagnosis of this life-threatening disease is problematic, because it...
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A Surge in the Seroprevalence of Toxoplasmosis among the Residents of Islands in Gangwha-gun, Incheon, Korea

Yang Z, Cho PY, Ahn SK, Ahn HJ, Kim TS, Chong CK, Hong SJ, Cha SH, Nam HW

Seroepidemiological changes of Toxoplasma gondii infection among the residents of the islands of Gangwha-gun, Incheon for 2 years were surveyed and evaluated by ELISA using a crude extract antigen. In...
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A Recombinant Plasmodium vivax Apical Membrane Antigen-1 to Detect Human Infection in Iran

Motevalli Haghi A, Khoramizade MR, Nateghpour M, Mohebali M, Edrissian , Eshraghian MR, Sepehrizadeh Z

In Iran, Plasmodium vivax is responsible for more than 80% of the infected cases of malaria per year. Control interventions for vivax malaria in humans rely mainly on developed diagnostic...
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