Korean J Parasitol.
2014 Apr;52(2):137-142.
Evaluation of Recombinant SAG1, SAG2, and SAG3 Antigens for Serodiagnosis of Toxoplasmosis
- Affiliations
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- 1Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. motazedm@sums.ac.ir
- 2Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
- 3Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
- 4Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abstract
- Serologic tests are widely accepted for diagnosing Toxoplasma gondii but purification and standardization of antigen needs to be improved. Recently, surface tachyzoite and bradyzoite antigens have become more attractive for this purpose. In this study, diagnostic usefulness of 3 recombinant antigens (SAG1, SAG2, and SAG3) were evaluated, and their efficacy was compared with the available commercial ELISA. The recombinant plasmids were transformed to JM109 strain of Escherichia coli, and the recombinants were expressed and purified. Recombinant SAG1, SAG2, and SAG3 antigens were evaluated using different groups of sera in an ELISA system, and the results were compared to those of a commercial IgG and IgM ELISA kit. The sensitivity and specificity of recombinant surface antigens for detection of anti-Toxoplasma IgG in comparison with commercially available ELISA were as follows: SAG1 (93.6% and 92.9%), SAG2 (100.0% and 89.4%), and SAG3 (95.4% and 91.2%), respectively. A high degree of agreement (96.9%) was observed between recombinant SAG2 and commercial ELISA in terms of detecting IgG anti-Toxoplasma antibodies. P22 had the best performance in detecting anti-Toxoplasma IgM in comparison with the other 2 recombinant antigens. Recombinant SAG1, SAG2, and SAG3 could all be used for diagnosis of IgG-specific antibodies against T. gondii.
Keyword
MeSH Terms
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Antibodies, Protozoan/*blood
Antigens, Protozoan/diagnostic use/*genetics
Enzyme-Linked Immunosorbent Assay
Humans
Immunoglobulin G/blood
Immunoglobulin M/blood
Membrane Glycoproteins/*genetics
Protozoan Proteins/*genetics
Recombinant Proteins/diagnostic use/immunology
Sensitivity and Specificity
Toxoplasma/immunology
Toxoplasmosis/blood/*diagnosis
Antibodies, Protozoan
Antigens, Protozoan
Immunoglobulin G
Immunoglobulin M
Membrane Glycoproteins
Protozoan Proteins
Recombinant Proteins
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