Int J Oral Biol.  2015 Mar;40(1):27-33. 10.11620/IJOB.2015.40.1.027.

Staurosporine Induces ROS-Mediated Process Formation in Human Gingival Fibroblasts and Rat Cortical Astrocytes

• Lee HG
• Kim DS
• Moon SA
• Kang JW
• Seo JT

• KMID: 1789366
• DOI: http://doi.org/10.11620/IJOB.2015.40.1.027

Abstract

In the present study, we investigated the effect of staurosporine on the formation of cellular processes in human gingival fibroblasts and rat astrocytes. Staurosporine caused a rapid induction of process formation in human gingival fibroblasts and rat astrocytes in a concentration dependent manner. The process formation of human gingival fibroblasts and rat astrocytes was prevented by the pretreatment with N-acetylcysteine, suggesting that staurosporine-induced ROS production was responsible for the process formation. Colchicine, a microtubule depolymerizing agent, inhibited the staurosporine-induced process formation, whereas cytochalasin D, an actin filament breakdown agent, failed to suppress the formation of cellular processes. This result indicated that polymerization of microtubule, and not actin filament, was responsible for the formation of cellular processes induced by staurosporine. In support of this hypothesis, Western blot analysis was conducted using anti-tubulin antibody, and the results showed that the amount of polymerized microtubule was increased by the treatment with staurosporine while that of depolymerized beta-tubulin in soluble fraction was decreased. These results indicate that staurosporine induces ROS-mediated, microtubule-dependent formation of cellular processes in human gingival fibroblasts and rat astrocytes.

Keyword

staurosporine; microtubule; human gingival fibroblasts; rat astrocytes

MeSH Terms

Acetylcysteine
Actin Cytoskeleton
Animals
Astrocytes*
Blotting, Western
Colchicine
Cytochalasin D
Fibroblasts*
Humans
Microtubules
Polymerization
Polymers
Rats*
Staurosporine*
Tubulin
Acetylcysteine
Colchicine
Cytochalasin D
Polymers
Staurosporine
Tubulin