Korean J Intern Med.  2013 Sep;28(5):579-586. 10.3904/kjim.2013.28.5.579.

Neuroprotective effects of Vitis vinifera extract on prediabetic mice induced by a high-fat diet

• Jin HY
• Cha YS
• Baek HS
• Park TS

• KMID: 1780106
• DOI: http://doi.org/10.3904/kjim.2013.28.5.579

Abstract

BACKGROUND/AIMS
Vitis vinifera grape seed extract (VVE) contains oligomeric proanthocyanidins that show antioxidant and free radical-scavenging activities. We evaluated VVE for its neuroprotective effect in prediabetic mice induce by a high-fat diet (HD).
METHODS
Mice were divided into four groups according to VVE dose: those fed a normal diet (ND; n = 10), HD (n = 10), HD with 100 mg/kg VVE (n = 10), and HD with 250 mg/kg VVE (n = 10). After 12 weeks, immunohistochemical analyses were carried out using a polyclonal antibody against antiprotein gene product 9.5 (protein-gene-product, 9.5), and intraepidermal innervation was subsequently quantified as nerve fiber abundance per unit length of epidermis (intraepidermal nerve fiber, IENF/mm).
RESULTS
Daily administration of VVE at doses of 100 or 250 mg/kg for 12 weeks protected HD mice from nerve fiber loss compared to untreated mice, as follows (IENF/mm): controls (40.95 +/- 5.40), HD (28.70 +/- 6.37), HD with 100 mg/kg (41.14 +/- 1.12), and HD with 250 mg/kg (48.98 +/- 7.01; p < 0.05, HD with VVE vs. HD).
CONCLUSIONS
This study provides scientific support for the therapeutic potential of VVE in peripheral neuropathy in an HD mouse model. Our results suggest that VVE could play a role in the management of peripheral neuropathy, similar to other antioxidants known to be beneficial for diabetic peripheral neuropathy.

Keyword

Antioxidants; Diet, high-fat; Peripheral nervous system diseases; Prediabetic state; Vitis vinifera grape seed extract

MeSH Terms

Animals
Antioxidants/*pharmacology
Biological Markers/blood
Blood Glucose/drug effects/metabolism
Body Weight/drug effects
Diabetic Neuropathies/blood/etiology/pathology/*prevention & control
*Diet, High-Fat
Disease Models, Animal
Dose-Response Relationship, Drug
Epidermis/*innervation
Grape Seed Extract/*pharmacology
Lipids/blood
Male
Mice
Mice, Inbred C57BL
Neuroprotective Agents/*pharmacology
Peripheral Nerves/*drug effects/pathology
Phytotherapy
Plants, Medicinal
Prediabetic State/blood/*drug therapy/etiology
Time Factors
*Vitis
Antioxidants
Biological Markers
Blood Glucose
Grape Seed Extract
Lipids
Neuroprotective Agents