J Vet Sci.
2014 Mar;15(1):51-60. 10.4142/jvs.2014.15.1.51.
Protective effects of silymarin on fumonisin B1-induced hepatotoxicity in mice
- Affiliations
-
- 1Department of Pathology, Samsun, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun 55139, Turkey. msozmen@hotmail.com
- 2Department of Biochemistry, Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, Burdur 15100, Turkey.
- 3Department of Pathology, Faculty of Veterinary Medicine, University of Adnan Menderes, Aydin 09010, Turkey.
- 4Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, Burdur 15100, Turkey.
- 5Department of Pathology, Faculty of Veterinary Medicine, University of Kafkas, Kars 36100, Turkey.
- 6Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Kirikkale, Kirikkale 71450, Turkey.
- KMID: 1737610
- DOI: http://doi.org/10.4142/jvs.2014.15.1.51
Abstract
- The present study was conducted to investigate the effect of silymarin on experimental liver toxication induced by Fumonisin B1 (FB1) in BALB/c mice. The mice were divided into six groups (n = 15). Group 1 served as the control. Group 2 was the silymarin control (100 mg/kg by gavage). Groups 3 and 4 were treated with FB1 (Group 3, 1.5 mg/kg FB1, intraperitoneally; and Group 4, 4.5 mg/kg FB1). Group 5 received FB1 (1.5 mg/kg) and silymarin (100 mg/kg), and Group 6 was given a higher dose of FB1 (4.5 mg/kg FB1) with silymarin (100 mg/kg). Silymarin treatment significantly decreased (p < 0.0001) the apoptotic rate. FB1 administration significantly increased (p < 0.0001) proliferating cell nuclear antigen and Ki-67 expression. Furthermore, FB1 elevated the levels of caspase-8 and tumor necrosis factor-alpha mediators while silymarin significantly reduced (p < 0.0001) the expression of these factors. Vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2) expressions were significantly elevated in Group 4 (p < 0.0001). Silymarin administration alleviated increased VEGF and FGF-2 expression levels (p < 0.0001). In conclusion, silymarin ameliorated toxic liver damage caused by FB1 in BALB/c mice.
MeSH Terms
-
Animals
Antioxidants/*pharmacology
Apoptosis/drug effects
Cell Proliferation/drug effects
Female
Fibroblast Growth Factor 2/genetics/metabolism
Fumonisins/*toxicity
Gene Expression Regulation/drug effects
Hepatocytes/*drug effects
Ki-67 Antigen/metabolism
Liver/drug effects
Mice
Mice, Inbred BALB C
Mycotoxins/*toxicity
Neovascularization, Physiologic/drug effects
Proliferating Cell Nuclear Antigen/metabolism
Silymarin/*pharmacology
Tumor Necrosis Factor-alpha/metabolism
Vascular Endothelial Growth Factor A/genetics/metabolism
Antioxidants
Fibroblast Growth Factor 2
Fumonisins
Ki-67 Antigen
Mycotoxins
Proliferating Cell Nuclear Antigen
Silymarin
Tumor Necrosis Factor-alpha
Vascular Endothelial Growth Factor A
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