Korean J Radiol.  2014 Aug;15(4):411-422. 10.3348/kjr.2014.15.4.411.

Trastuzumab-Conjugated Liposome-Coated Fluorescent Magnetic Nanoparticles to Target Breast Cancer

Affiliations
  • 1Department of Radiology, Seoul National University Bundang Hospital, Seongnam 463-707, Korea. kimsmlms@daum.net
  • 2Nanoimaging and Therapy Research Center, Institute of Nanoconvergence, Advanced Institutes of Convergence Technology, Seoul National University, Suwon 443-270, Korea.
  • 3NanoBio Materials Chemistry Lab., Department of Applied Bioscience, CHA University, Pocheon 487-010, Korea.
  • 4Program in Nano Science and Technology, Department of Transdisciplinary Studies, Seoul National University Graduate School of Convergence Science and Technology, Suwon 443-270, Korea.

Abstract


OBJECTIVE
To synthesize mesoporous silica-core-shell magnetic nanoparticles (MNPs) encapsulated by liposomes (Lipo [MNP@m-SiO2]) in order to enhance their stability, allow them to be used in any buffer solution, and to produce trastuzumab-conjugated (Lipo[MNP@m-SiO2]-Her2Ab) nanoparticles to be utilized in vitro for the targeting of breast cancer.
MATERIALS AND METHODS
The physiochemical characteristics of Lipo[MNP@m-SiO2] were assessed in terms of size, morphological features, and in vitro safety. The multimodal imaging properties of the organic dye incorporated into Lipo[MNP@m-SiO2] were assessed with both in vitro fluorescence and MR imaging. The specific targeting ability of trastuzumab (Her2/neu antibody, Herceptin(R))-conjugated Lipo[MNP@m-SiO2] for Her2/neu-positive breast cancer cells was also evaluated with fluorescence and MR imaging.
RESULTS
We obtained uniformly-sized and evenly distributed Lipo[MNP@m-SiO2] that demonstrated biological stability, while not disrupting cell viability. Her2/neu-positive breast cancer cell targeting by trastuzumab-conjugated Lipo[MNP@m-SiO2] was observed by in vitro fluorescence and MR imaging.
CONCLUSION
Trastuzumab-conjugated Lipo[MNP@m-SiO2] is a potential treatment tool for targeted drug delivery in Her2/neu-positive breast cancer.

Keyword

Breast cancer; Drug delivery; Iron oxide nanoparticles; Magnetic resonance imaging; Trastuzumab

MeSH Terms

3T3 Cells
Animals
Antibodies, Monoclonal, Humanized/*administration & dosage
Antineoplastic Agents/*administration & dosage
Breast Neoplasms/chemistry/*drug therapy
Cell Line, Tumor
Drug Delivery Systems/methods
Female
Ferric Compounds/chemistry
Humans
Liposomes
Magnetite Nanoparticles/administration & dosage/*chemistry
Mice
Molecular Targeted Therapy/methods
Nanoconjugates/administration & dosage/*chemistry
Nanoparticles/chemistry
*Receptor, erbB-2/immunology
Silicon Dioxide/administration & dosage/*chemical synthesis/chemistry
Antibodies, Monoclonal, Humanized
Antineoplastic Agents
Ferric Compounds
Liposomes
Magnetite Nanoparticles
Nanoconjugates
Silicon Dioxide
Receptor, erbB-2
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