J Vet Sci.
2013 Sep;14(3):345-353. 10.4142/jvs.2013.14.3.345.
Black rice anthocyanidins prevent retinal photochemical damage via involvement of the AP-1/NF-kappaB/Caspase-1 pathway in Sprague-Dawley Rats
- Affiliations
-
- 1Department of Public Health, Chengdu Medical College, Chengdu City, 610050, China. cyggwsyxp@sina.com
- 2Department of Nutrition and Food Hygiene, School of Preventive Medicine, The Third Military Medical University, Chongqing City, 400038, China.
- 3Department of Epidemiology and Bio-statistics, School of Public Health, Drexel University, Philadelphia, PA 19102, USA.
- KMID: 1705566
- DOI: http://doi.org/10.4142/jvs.2013.14.3.345
Abstract
- The effects of black rice anthocyanidins (BRACs) on retinal damage induced by photochemical stress are not well known. In the present study, Sprague-Dawley rats were fed AIN-93M for 1 week, after which 80 rats were randomly divided into two groups and treated with (n = 40) or without BRACs (n = 40) for 15 days, respectively. After treatment, both groups were exposed to fluorescent light (3,000 +/- 200 lux; 25degrees C), and the protective effect of dietary BRACs were evaluated afterwards. Our results showed that dietary BRACs effectively prevented retinal photochemical damage and inhibited the retinal cells apoptosis induced by fluorescent light (p < 0.05). Moreover, dietary BRACs inhibited expression of AP-1 (c-fos/c-jun subunits), up-regulated NF-kappaB (p65) expression and phosphorylation of IkappaB-alpha, and decreased Caspase-1 expression (p < 0.05). These results suggest that BRACs improve retinal damage produced by photochemical stress in rats via AP-1/NF-kappaB/Caspase-1 apoptotic mechanisms.
MeSH Terms
-
Animal Feed/analysis
Animals
Anthocyanins/administration & dosage/*pharmacology
Antioxidants/administration & dosage/*physiology
Blotting, Western
Caspase 1/*genetics/metabolism
Diet
Dietary Supplements/analysis
I-kappa B Proteins/genetics/metabolism
NF-kappa B/*genetics/metabolism
Neoplasm Proteins/genetics/metabolism
Nucleocytoplasmic Transport Proteins/genetics/metabolism
Oryza sativa/chemistry
Proto-Oncogene Proteins c-fos/genetics/metabolism
Proto-Oncogene Proteins c-jun/genetics/metabolism
Rats
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Retinal Diseases/etiology/*prevention & control
Signal Transduction/*drug effects/radiation effects
Transcription Factor AP-1/*genetics/metabolism
Anthocyanins
Antioxidants
Caspase 1
I-kappa B Proteins
NF-kappa B
Neoplasm Proteins
Nucleocytoplasmic Transport Proteins
Proto-Oncogene Proteins c-fos
Proto-Oncogene Proteins c-jun
Transcription Factor AP-1
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- NF-kappa B activation following optic nerve transection
- PDTC Inhibits TNF-alpha-Induced Apoptosis in MC3T3E1 Cells
- The Impact of Kinds of Dietary Grain and Dietary Lipid Level on the Glucose Metabolism and Antithrombogenic Capacity of Full Grown Obesity Induced Rats
- Alteration of PKC, PKC theta, NF-kappa B and AP-1 in Ischemic-reperfused Soleus Muscle of Rat with Ischemic Preconditioning
- NF-kappaB Binding Activity and Cyclooxygenase-2 Expression in Persistent betaCCI(4)-Treated Rat Liver Injury
