J Biomed Res.
2013 Dec;14(4):240-248.
Anti-proliferative efficacy comparison of conjugated linoleic acid on human cancer cell lines
- Affiliations
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- 1Division of Applied Life Science (BK21 Plus), Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 660-701, Korea. ylha@gnu.kr
- 2Department of Oriental Medicine, Daegu Haany University, Gyeongsan 712-715, Korea.
- 3Department of Internal medicine, Gyeongnam Regional Cancer Center, and Institute of Health Science, Gyeongsang National University School of Medicine, Jinju 660-702, Korea.
- 4School of Food Science, International University of Korea, Jinju 660-759, Korea.
- 5Medical School, Gyeongsang National University, Jinju 660-702, Korea.
- 6Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea.
- 7Laboratory of Biochemistry (BK21 Plus), School of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Korea.
Abstract
- The anti-proliferative efficacy of t,t-conjugated linoleic acid (t,t-CLA), c9,t11-CLA, and t10,c12-CLA was compared in several human cancer cell lines. Gastric NCI-N87, liver Hep3B, pancreas Capan-2, and lung NCI-H522 cancer cells were incubated with 50 microM CLA isomers over a period of 6 days. The t,t-CLA inhibited the growth of all cancer cell lines to different extents, but c9,t11-CLA and t10,c12-CLA inhibited or stimulated the growth of the cancer cell lines. NCI-N87 cells were the most sensitive to growth inhibition and apoptosis from all CLA isomers tested. In NCI-N87 cells, CLA isomers reduced the release of arachidonic acid (AA) via the inhibition of cytosolic phospholipase A2 (cPLA2) activity, consequently reducing the production of PGE2 through the inhibition of cyclooxygenase-2 (COX-2). The efficacies of CLA isomers were in the following order (from most to least effective): t,t-CLA, t10,c12-CLA and c9,t11-CLA. Overall, these results imply that the anti-proliferative efficacy of t,t-CLA on cancer cells, especially NCI-N87 cells, was greater than other CLA isomers due to its induction of apoptosis through the inhibition of cPLA2 and COX-2 activities.
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