Korean J Phys Anthropol.  2005 Dec;18(4):283-290.

Micro-CT Evaluation in Osteoporosis Model

• Ko CY
• Woo DG
• Kim HS
• Lee BY

Abstract

Osteoporosis is a major health-care problem for aging communities. And postmenopausal osteoporosis is a common disorder characterized by an increase in bone resorption relative to bone formation, generally in conjunction with an increased rate of bone turnover. Twenty female rats were used as rat model of osteoporosis. They randomly were divided into two groups: a normal control group, and calcium-free diet groups treated with bilateral ovariectomy. The animals were sacrified at 12 weeks after treatment. In the rats with osteoporosis, there was significant thining and loss of trabeculae, accompanied by a disconnection of trabeculae. And acid mucopolysaccharides were decreased in osteoporotic animals. In order to setup conditions of invivo micro-CT, proper anesthetic is ketamine (50 mg/kg) /xylazine (6 mg/kg). And proper breathing rate of experimental animals is 1.3~1.6/sec. Our finding suggest that bilateral ovariectomy and calcium-free diet induce decrease in thickeness of trabecular bone and decrease in acid mucopolysaccharide, therefore cause weakness of bone. And micro-CT imaging system is useful to evaluate trabecular bone. Anesthetic time, anesthetics and respiration rate also signify much in micro-CT imaging system.

Keyword

In-vivo; Micro-CT; Osteoporosis; Trabecular bone; Ovariectomy

MeSH Terms

Aging
Anesthetics
Animals
Bone Resorption
Diet
Female
Glycosaminoglycans
Humans
Ketamine
Models, Animal
Osteogenesis
Osteoporosis*
Osteoporosis, Postmenopausal
Ovariectomy
Rats
Respiration
Respiratory Rate
Anesthetics
Glycosaminoglycans
Ketamine