Korean J Otolaryngol-Head Neck Surg.  2005 May;48(5):606-614.

Membrane-Specific Expression of Functional Purinergic Receptors in Normal Human Nasal Epithelial Cells

• Kim CH
• Kim SS
• Yoo JB
• Lee SY
• Song MH
• Lee JG
• Yoon JH

Abstract

BACKGROUND AND OBJECTIVES
Extracellular purines and pyrimidines regulate various physiological responses via cell surface receptors known as purinoreceptors, and may exert autocrine or paracrine effects on ion transport, fluid transport, ciliary beat frequency and mucin secretion. This study aims to investigate the expression patterns of such purinoreceptors found in normal human nasal epithelial (NHNE) cells. MATERIALS AND METHOD: In RT-PCR, the mRNAs for several P2X (P2X3, P2X4, P2X7) and P2Y (P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12) receptors were identified in NHNE cells. Functional localizations of P2 receptors were investigated by measuring [Ca2+]i increases in a membrane-specific manner using a double-perfusion chamber. Absence of the responses of -Me ATP and 2MeS-ATP excluded functionally active P2X3, P2X4, and P2Y1 receptors as far as [Ca2+]i increase was concerned. RESULTS: Applications with ATP and UTP revealed that luminal membranes of NHNE cells express P2Y2 and P2Y6 receptors and basolateral membranes P2Y2 receptors. Expressions of P2Y2 and P2Y6 receptors in NHNE cells were further verified by the immunoblotting using specific antibodies. In addition, the results with BzATP indicated that the P2Y11 receptor may be present on the luminal side. CONCLUSION: The NHNE cells express functionally active P2Y2, P2Y6 and P2Y11 receptors in a membrane-specific pattern, which may play an important role in the control of mucin and fluid secretion in NHNE cells.

Keyword

Purinoreceptor; Calcium; Human; Nasal mucosa; Secretions

MeSH Terms

Adenosine Triphosphate
Antibodies
Calcium
Epithelial Cells*
Humans*
Immunoblotting
Ion Transport
Membranes
Mucins
Nasal Mucosa
Phenobarbital
Purines
Pyrimidines
Receptors, Cell Surface
Receptors, Purinergic P2Y1
Receptors, Purinergic P2Y2
Receptors, Purinergic*
RNA, Messenger
Uridine Triphosphate
Adenosine Triphosphate
Antibodies
Calcium
Mucins
Phenobarbital
Purines
Pyrimidines
RNA, Messenger
Receptors, Cell Surface
Receptors, Purinergic
Receptors, Purinergic P2Y1
Receptors, Purinergic P2Y2
Uridine Triphosphate