Korean J Dermatol.
2001 Apr;39(4):407-412.
Use of Human Skin Reconstructs Containing Melanocytes as a Model to Evaluate the Effect of Pigment Modifiers
- Affiliations
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- 1Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea. khcho@snu.ac.kr
Abstract
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BACKGROUND: Recently human skin reconstructs composed of keratinocytes and melanocytes have been developed.
OBJECTIVE
We investigated whether human skin reconstructs containing melanocytes could be useful to test the efficacy of drugs that alter pigmentation.
METHODS
Human skin reconstructs were made by culturing human keratinocytes and melanocytes on the top of de-epidermized dermis. Reagents that alter melanogenesis such as isobutyl methylxanthine, 1-oleoyl-2-acetyl-sn-glycerol, kojic acid were treated in the culture medium after 7 days of culture at the air-liquid interface. Macroscopic, histological, histochemical and immunohistochemical studies were performed.
RESULTS
The effect of pigment modifiers was demonstrated in human skin reconstructs as expected from in vivo data on skin color, numbers of melanocytes and melanin content.
CONCLUSION
These results indicate that this model is very useful to assess the efficacy of drugs that alter pigmentation.
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