J Korean Soc Ther Radiol Oncol.  2005 Sep;23(3):161-168.

The Change of Transforming Growth Factor beta1 (TGF-beta1) Expression by Melatonin in Irradiated Lung

Affiliations
  • 1Department of Radiation Oncology, College of Medicine, The Catholic University of Korea, Seoul, Korea. ssjang@catholic.ac.kr

Abstract

PURPOSE: The changed expressions of TGF-beta1, as a key cytokine in the fibrotic process, due to melatonin with potent antioxidative effects, were investigated in the irradiated lung using fibrosis-sensitive C57BL/6 mice.
MATERIALS AND METHODS
Female C57BL/6 mice were divided into control irradiation-only, and melatonin (300 mg/kg i.p. 1 hr before irradiation) pretreatment groups. The thoraces of the mice were irradiated with a single dose of 12 Gy. The mRNA expressions of TGF-beta1 in the lung tissue 2 and 4 weeks after irradiation were quantified using semiquantitive RT-PCR, and the cellular origin and expression levels of TGF-beta1 protein were identified using immunohistochemical staining.
RESULTS
The relative mRNA expression levels in the irradiation-only and melatonin pretreatment groups 2 and 4 weeks after irradiation were 1.92- and 1.80-fold (p=0.064) and 2.38- and 1.94-fold (p=0.004) increased, respectively compared to those in the control group. Increased expressions of TGF-beta1 protein were prominently detected in regions of histopathological radiation injury, with alveolar macrophages and septal epithelial cells serving as important sources of TGF-beta1 expression. At 2 and 4 weeks after irradiation, the expression levels of protein were 15.8% vs. 16.9% (p=0.565) and 36.1% vs. 25.7% (p=0.009), respectively.
CONCLUSION
The mRNA and protein expressions of TGF-beta1 in the lung tissue following thoracic irradiation with 12 Gy were significantly decreased by melatonin pretreatment at 4 weeks. These results indicate that melatonin may have a possible application as an antifibrotic agent in radiation-induced lung injury.

Keyword

Melatonin; TGF-beta1; Radiation; Fibrosis

MeSH Terms

Animals
Epithelial Cells
Female
Fibrosis
Humans
Lung Injury
Lung*
Macrophages, Alveolar
Melatonin*
Mice
Radiation Injuries
RNA, Messenger
Thorax
Transforming Growth Factor beta1*
Transforming Growth Factors*
Melatonin
RNA, Messenger
Transforming Growth Factor beta1
Transforming Growth Factors
Full Text Links
  • JKSTRO
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr