Korean J Physiol Pharmacol.  2006 Dec;10(6):309-315.

Baicalein Protects 6-OHDA-induced Neuronal Damage by Suppressing Oxidative Stress

• Im HI
• Nam E
• Lee ES
• Hwang YJ
• Kim YS

Abstract

The protective effects of baicalein, one of the flavonoids in Scutellaria baicalensis Georgi, were evaluated against 6-hydroxydopamine (6-OHDA)-induced neuronal damage in mice and cultured human neuroblastoma cells. Nigrostriatal damage was induced by stereotaxically injecting 6-OHDA into the right striatum. Baicalein was administered intraperitoneally 30 min before and 90 min after lesion induction. Animals received a further daily injection of baicalein for 3 consecutive days. Two weeks after 6-OHDA injection, contralateral rotational asymmetry was observed by apomorphine challenge in lesioned mice. Tyrosine hydroxylase (TH) immunohistochemistry revealed a significant loss of terminals in lesioned striatum and the reduction of the numbers of TH-positive cell in the ipsilateral substantia nigra (SN). In addition, the levels of dopamine (DA) and DA metabolites were reduced and lipid peroxidation was increased in lesioned striatum. However, baicalein treatment reduced apomorphine-induced rotational behavior in 6-OHDA-lesioned mice, and increased TH immunoreactivity in the striatum and SN, and DA levels in lesioned striatum. Lipid peroxidation induced by 6-OHDA was also inhibited by baicalein treatment. Furthermore, when SH-SY5Y human neuroblastoma cells were treated with baicalein, 6-OHDA-induced cytotoxicity and reactive oxygen species (ROS) production were significantly reduced. These results indicate that baicalein effectively protects 6-OHDA-induced neuronal damage through antioxidant action.

Keyword

6-OHDA; Baicalein; Tyrosine hydroxylase; Dopamine; Oxidative stress; Antioxidant

MeSH Terms

Animals
Apomorphine
Dopamine
Flavonoids
Humans
Immunohistochemistry
Lipid Peroxidation
Mice
Neuroblastoma
Neurons*
Oxidative Stress*
Oxidopamine
Reactive Oxygen Species
Scutellaria baicalensis
Substantia Nigra
Tyrosine 3-Monooxygenase
Apomorphine
Dopamine
Flavonoids
Oxidopamine
Reactive Oxygen Species
Tyrosine 3-Monooxygenase