J Genet Med.  1998 Mar;2(1):31-34.

Tissue-specific Expression of DNA Repair Gene, N-Methylpurine-DNA Glycosylase (MPG) in Balb/c Mice without External Damage

• Kim NK

Abstract

The N-methylpurine-DNA glycosylase (MPG), a ubiquitous DNA repair enzyme, removes N-methylpurine and other damaged purines induced in DNA. Tissue-specific mRNA levels of the N-methylpurine-DNA glycosylase (MPG) were investigated in Balb/c mice of four different growing stages; newborn, 1, 4 and 8-weeks postpartum. MPG expressions in the newborn and the 8-week-old mice were the highest in thymus and testis, respectively. The tested tissues of the newborn mice had consistently higher MPG mRNA level than 8-week-old adults except in testis and thymus. The MPG mRNA level in testis was the lowest in the newborn mice, but it attained the highest in the 8-week-old mice. The levels of MPG mRNA among the different tissues in the newborn and the 8-week-old mice were more than 9.0 and 19.0-fold respectively. These results suggest that the of MPG expression was dependent on the growing stage and had tissue-specificity.

Keyword

DNA repair; gene expression; mouse; MPG; tissue-specificity

MeSH Terms

Adult
Animals
DNA Repair*
DNA*
Gene Expression
Humans
Infant, Newborn
Mice*
Postpartum Period
Purines
RNA, Messenger
Testis
Thymus Gland
DNA
Purines
RNA, Messenger