Korean J Anat.  2006 Dec;39(6):523-531.

A Study of Immunohistochemical Change of the Experimental Ulcerative Colitis induced by DSS

• Park IS

Abstract

The experimental ulcerative colitis is chronic inflammatory illness in colon, which didn't reveal the exact reason and pathophysiological situation. In this study, the colitis was induced by 3% DSS (mw; 40,000) for 5 days in mouse which was resemble to inflammatory bowel disease in human, and immunohistochemical changes were observed in the mucosa. In inflammatory group, thickness of the colon was increased and the length of colon was shorter than that of the normal group and the body weight decreased. In microscopic aspect, the crypt erosion, many inflammatory cells and submucosal edema were occurred. In immunohistochemical study, the immunity of COX1 in the inflammatory group was not changed to comparing the normal group, but COX2 immunoreactivity was increased than the normal group, HSP70 immunoreactivity were also increased than the normal group. MAC387 which used to detect the macrophage was increased than the normal group and PCNA immunoreactivity were increased along to the mucal layer. And the number of apoptosis cells detected by TUNEL was increased. In these results, the experimental colitis revealed the tissue defense mechanism as well as inflammatory system and observed the stuffs related to the regeneration.

Keyword

Colitis; DSS (dextran sulfate sodium); Inflammation; Immunohistochemistry; Apoptosis

MeSH Terms

Animals
Apoptosis
Body Weight
Colitis
Colitis, Ulcerative*
Colon
Edema
Humans
Immunohistochemistry
In Situ Nick-End Labeling
Inflammation
Inflammatory Bowel Diseases
Macrophages
Mice
Mucous Membrane
Proliferating Cell Nuclear Antigen
Regeneration
Ulcer*
Proliferating Cell Nuclear Antigen