J Korean Assoc Oral Maxillofac Surg.  2009 Aug;35(4):199-204.

Xenografted Tumorigenesis in the oral vestibule of nude mice by Snail transfection: Histological and immunohistochemical study

• Kim MK
• Lee EH
• Kim J
• Yook JI
• Cha IH

Abstract

PURPOSE
The purpose of this study is to investigate the epithelial-mesenchymal transition (EMT) induced by Snail transcription factor and Snailtransfected in vivo tumors with histopathological features.
MATERIALS AND METHODS
We induced in vivo xenografted tumorigenesis in the oral vestibules of nude mice by a Snail transfected HaCaT cell line and investigated morphological and immunohistochemical features in Snail expressive tumors.
RESULTS
We identified tumor masses in 14 out of 15 nude mice in the HaCaT-Snail cell inoculation group, but no tumors were present in any of the HaCaT cell inoculation group. Induced tumors showed features of poorly differentiated carcinoma with invasion to neighboring muscles and bones. The HaCaT-Snail tumors showed decreased expressions of E-cadherin and cytokeratin, but showed increased expressions of vimentin and N-cadherin. DISCUSSION: The Snail transfected xenograft can improve productivity of malignant tumors, show various histopathological features including invasive growth, and aid in the investigation of tumor progression and the interaction with surrounding tissues.

Keyword

E-cadherin; neoplastic cell transformation; Snail transcription factor; xenograft model

MeSH Terms

Animals
Cadherins
Cell Line
Cell Transformation, Neoplastic
Efficiency
Epithelial-Mesenchymal Transition
Keratins
Mice
Mice, Nude
Muscles
Snails
Transcription Factors
Transplantation, Heterologous
Vimentin
Cadherins
Keratins
Transcription Factors
Vimentin