Korean J Obstet Gynecol.  2007 Nov;50(11):1493-1498.

Inhibition of clusterin gene expression via shRNA increases chemosensitivity to paclitaxel in xenografted PEOH cells

• Shin EY
• Jeung IC
• Choi JH
• Park DC

Abstract

OBJECTIVE
To evaluate the inhibition of Clusterin gene expression via shRNA decreases proliferation and metastasis and increases chemosensitivity to paclitaxel in xenografted PEOH cells.
METHODS
1 x 10(6) paclitaxel resistant cell lines transduced with Clusterin shRNA in lentiviral inoculated subcutaneously into the flank region of 6 to 8 week-old female nude mice. Parental cells transduced with LacZ was used as a control. Tumor growth was measured twice every week and calculated by using the formula: length x width x depth x 0.5236. The mice were sacrificed and examined for Clusterin expression on tumor cells and counted the metastasis sites.
RESULTS
shRNA for Cluaterin works in vivo and it is the in accord with the in vitro results. Although shRNA for Clusterin group showed decreased tumor growth and proliferation it has not statistical significance. But transfection of Clusterin shRNA on PEOH significantly increased paclitaxel-sensitivity (P<0.05).
CONCLUSION
shRNA targeting of the Clusterin gene decreased the ovarian cancer cell's paclitaxel resistance.

Keyword

Clusterin; Ovarian cancer; shRNA; Paclitaxel resistance

MeSH Terms

Animals
Cell Line
Clusterin*
Female
Gene Expression*
Heterografts*
Humans
Mice
Mice, Nude
Neoplasm Metastasis
Ovarian Neoplasms
Paclitaxel*
Parents
RNA, Small Interfering*
Transfection
Clusterin
Paclitaxel
RNA, Small Interfering