Maxillofac Plast Reconstr Surg.  2014 Mar;36(2):50-56.

Bone Induction by Demineralized Dentin Matrix in Nude Mouse Muscles

• Kim KW

Abstract

PURPOSE
This study examined the osteoinductive activity of demineralized human dentin matrix for nude mice.
METHODS
Twenty healthy nude mice weighing about 15 to 20 g were used for study. Demineralized human dentin matrix was prepared and implanted into the dorsal portion of nude mice (subcutaneous), which were sacrificed at two, four, and eight weeks after demineralized dentin matrix grafting and evaluated histologically by H&E and Masson trichrome staining. The specimens were also evaluated histomorphometrically.
RESULTS
The demineralized dentin matrix induced bone and cartilage formation independently in soft tissues. Histological examination showed bone-forming cells such as osteoblasts and fibroblasts at two, four, and eight weeks.
CONCLUSION
These results suggest that demineralized human dentin matrix has osteoinductive ability, and is a good alternative to autogenous bone graft materials.

Keyword

Dentin; Demineralized human dentin; Bone induction

MeSH Terms

Animals
Cartilage
Dentin*
Fibroblasts
Humans
Mice
Mice, Nude*
Muscles*
Osteoblasts
Transplants