Maxillofac Plast Reconstr Surg.  2016 Feb;38(2):7. 10.1186/s40902-016-0053-5.

Histologic study of bone-forming capacity on polydeoxyribonucleotide combined with demineralized dentin matrix

• Kim SK
• Huh CK
• Lee JH
• Kim KW
• Kim MY

• KMID: 2158580
• DOI: http://doi.org/10.1186/s40902-016-0053-5

Abstract

BACKGROUND
This study examined the osteoinductive activity of demineralized dentin matrix (DDM) from human and polydeoxyribonucleotide (PDRN) for nude mice.
METHODS
Twenty healthy nude mice, weighing about 15~20 g, were used for the study. DDM from human and PDRN were prepared and implanted subcutaneously into the dorsal portion of the nude mice. The nude mice were sacrificed at 1, 2, and 4 weeks after grafting and evaluated histologically by hematoxylin-eosin and Masson's trichrome staining. The specimens were also evaluated via a histomorphometric study.
RESULTS
The DDM and PDRN induced new bone, osteoblasts, and fibroblasts in soft tissues. The histological findings showed bone-forming cells like osteoblasts and fibroblasts at 1, 2, and 4 weeks. New bone formation was observed in the histomorphometric study. In particular, the ratio of new bone formation was the highest at 2 weeks compared with the first week and fourth week.
CONCLUSIONS
In this study, we showed that the PDRN used in this experimental model was able to induce bone regeneration when combined to the DDM.

Keyword

Polydeoxyribonucleotide (PDRN); Demineralized dentin matrix (DDM); Osteoinduction; Nude mice

MeSH Terms

Animals
Bone Regeneration
Dentin*
Fibroblasts
Humans
Mice
Mice, Nude
Models, Theoretical
Osteoblasts
Osteogenesis
Transplants