Korean J Pathol.
2011 Oct;45(5):469-476. 10.4132/KoreanJPathol.2011.45.5.469.
CpG Island Methylation According to the Histologic Patterns of Early Gastric Adenocarcinoma
- Affiliations
-
- 1Department of Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea. meeyon@yonsei.ac.kr
- 2Department of Internal Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
- KMID: 1443696
- DOI: http://doi.org/10.4132/KoreanJPathol.2011.45.5.469
Abstract
- BACKGROUND
Although the importance of aberrant DNA methylation in the development of gastric adenocarcinoma has been described, the mechanism of pathogenesis has not been revealed yet. We quantitatively analyzed methylation of four CpG islands and one repetitive DNA element, according to the histologic features of adenocarcinoma with precursor lesions.
METHODS
We divided the cases as adenocarcinoma with intestinal type precursors (type A, n=19 cases) and adenocarcinoma with diffuse type precursors (type B, n=19 cases). We micro-dissected tumor cells and matched non-neoplastic gastric mucosa from the hematoxylin and eosin-stained slides.
RESULTS
A total of 20 CpG sites of long interspersed nucleotide element-1 (LINE1), RAR-related orphan receptor alpha (RORA), Kruppel-like factor 7 (KLF7), mutL homolog 1 (MLH1), MINT25, and CD133 were analyzed. Methylation was determined by bisulfate-pyro-sequencing, and hypomethylation of LINE1 and CD133 was noted in the tumors, compared to the levels in the non-neoplastic gastric mucosa (p=0.014 and p=0.015, respectively). A statistically different methylation pattern of CpG sites at CD133 and KLF7 was noted only in type B lesions, compared to that in matched non-neoplastic gastric mucosa (p=0.027 and p=0.043, respectively).
CONCLUSIONS
Given that aberrant methylation occurs in a relatively early phase of carcinogenesis, different patterns of methylation may determine the carcinoma phenotype. However, further large-scale study is required to clarify the significance of this difference.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- CpG Island Hypermethylation in Gastric Carcinoma and Its Premalignant Lesions
- The Relationship between the Methylenetetrahydrofolate Reductase Genotypes and the Methylation Status of the CpG Island Loci, LINE-1 and Alu in Prostate Adenocarcinoma
- DNA Methylation Changes in Human Cancers
- DNA Methylation as Surrogate Marker For Gastric Cancer
- CpG island methylation and gynecolgic malignancy
