Tuberc Respir Dis.  2011 Mar;70(3):218-223. 10.4046/trd.2011.70.3.218.

Effects of Curcumin and Genistein on Phorbol Ester or Tumor Necrosis Factor-alpha-Induced Mucin Production from Human Airway Epithelial Cells

• Lee SY
• Lee HJ
• Lee JW
• Jeon BK
• Kim JO
• Lee CJ

• KMID: 1442851
• DOI: http://doi.org/10.4046/trd.2011.70.3.218

Abstract

BACKGROUND
We investigated whether curcumin and genistein affect the MUC5AC mucin production from human airway epithelial cells that is induced by phorbol 12-myristate 13-acetate (PMA) or tumor necrosis factor-alpha (TNF-alpha).
METHODS
Confluent NCI-H292 cells were pretreated with each agent for 30 min and then stimulated with PMA or TNF-alpha for 24 hours. MUC5AC mucin production was measured by an ELISA.
RESULTS
(1) Curcumin dose-dependently inhibited the production of MUC5AC mucin that was induced by PMA or TNF-alpha; (2) Genistein inhibited PMA-induced MUC5AC mucin production. However, it did not decrease TNF-alpha-induced MUC5AC mucin production.
CONCLUSION
These results suggest that curcumin and genistein inhibit the production of airway mucin induced by PMA.

Keyword

Mucin 5AC; Curcumin; Genistein

MeSH Terms

Curcumin
Epithelial Cells
Genistein
Humans
Mucin 5AC
Mucins
Necrosis
Phorbols
Tumor Necrosis Factor-alpha
Curcumin
Genistein
Mucin 5AC
Mucins
Phorbols
Tumor Necrosis Factor-alpha

Figure

• 

  Figure 1 Effect of curcumin on PMA-induced MUC5AC mucin production from NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of curcumin for 30 min and then stimulated with PMA (10 ng/mL) for 24 hours. Cell lysates were collected for measurement of MUC5AC mucin production by ELISA. Means of individual group were converted to percent control and expressed as mean±SEM. The difference between groups was assessed using one-way ANOVA and Student's t-test for unpaired samples. p<0.05 was considered as significantly different. Each bar represents a mean±SEM. of 3~4 culture wells in comparison with that of a control set at 100%. *Significantly different from control (p<0.05), †Significantly different from PMA alone (p<0.05). SEM: standard error of the mean; cont: control; C: curcumin, concentration unit is µM.; PMA: phorbol 12-myristate 13-acetate.

• 

  Figure 2 Effect of curcumin on TNF-α-induced MUC5AC mucin production from NCI-H292 cells. NCI-H292 cells were pretreated with various concentrations of curcumin for 30min and then stimulated with TNF-α (0.2 nM) for 24 hours. Cell lysates were collected for measurement of MUC5AC mucin production by ELISA. Means of individual group were converted to percent control and expressed as mean±SEM. The difference between groups was assessed using one-way ANOVA and Student's t-test for unpaired samples. p<0.05 was considered as significantly different. Each bar represents a mean±SEM. of 3~4 culture wells in comparison with that of control set at 100%. *Significantly different from control (p<0.05), †Significantly different from TNF-α alone (p<0.05). SEM: standard error of the mean; cont: control; C: curcumin, concentration unit is µM.; PMA: phorbol 12-myristate 13-acetate; TNF: tumor necrosis factor.

• 

  Figure 3 Effect of genistein on PMA-induced MUC5AC mucin production from NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of genistein for 30 min and then stimulated with PMA (10 ng/mL) for 24 hours. Cell lysates were collected for measurement of MUC5AC mucin production by ELISA. Means of individual group were converted to percent control and expressed as mean±SEM. The difference between groups was assessed using one-way ANOVA and Student's t-test for unpaired samples. p<0.05 was considered as significantly different. Each bar represents a mean±SEM of 3~4 culture wells in comparison with that of a control set at 100%. *Significantly different from control (p<0.05), †Significantly different from PMA alone (p<0.05). SEM: standard error of the mean; cont: control; PMA: phorbol 12-myristate 13-acetate; G: genistein, concentration unit is µM.

• 

  Figure 4 Effect of genistein on TNF-α-induced MUC5AC mucin production from NCI-H292 cells. NCI-H292 cells were pretreated with various concentrations of genistein for 30 min and then stimulated with TNF-α (0.2 nM) for 24 hours. Cell lysates were collected for measurement of MUC5AC mucin production by ELISA. Means of individual group were converted to percent control and expressed as mean±SEM. The difference between groups was assessed using one-way ANOVA and Student's t-test for unpaired samples. p<0.05 was considered as significantly different. Each bar represents a mean±SEM. of 3~4 culture wells in comparison with that of control set at 100%. *Significantly different from control (p<0.05). ELISA: enzyme-linked immunosorbent assay; cont: control; G: genistein, concentration unit is µM.

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