Nat Prod Sci.  2019 Sep;25(3):248-254. 10.20307/nps.2019.25.3.248.

Growth Factor- and Phorbol Ester-induced Production and Gene Expression of MUC5AC Mucin in Human Airway Epithelial NCI-H292 Cells Were Inhibited by Afzelin and Natural Products Derived from Houttuynia Cordata

• Kim YJ
• Lee HJ
• Lee CJ

• KMID: 2459968
• DOI: http://doi.org/10.20307/nps.2019.25.3.248

Abstract

In the present study, we investigated whether quercitrin, quercetin and afzelin derived from Houttuynia cordata affect the production and gene expression of MUC5AC mucin from airway epithelial cells. Confluent NCI-H292 cells were pretreated with quercitrin, quercetin or afzelin for 30 min and then stimulated with epidermal growth factor (EGF) or phorbol 12-myristate 13-acetate (PMA) for 24 h. The MUC5AC mucin gene expression and production were measured by RT-PCR and ELISA, respectively. The results were as follows: (1) Quercitrin, quercetin and afzelin inhibited EGF- and PMA-induced MUC5AC mucin production from NCI-H292 cells; (2) The three natural products also decreased EGF- and PMA-induced MUC5AC mucin gene expression in NCI-H292 cells. These results suggest that quercitrin, quercetin and afzelin showed the regulatory effect on the steps of gene expression and production of mucin, by directly acting on airway epithelial cells.

Keyword

airway; mucin; natural products

MeSH Terms

Biological Products*
Enzyme-Linked Immunosorbent Assay
Epidermal Growth Factor
Epithelial Cells
Gene Expression*
Houttuynia*
Humans*
Mucins*
Quercetin
Biological Products
Epidermal Growth Factor
Mucins
Quercetin

Figure

• 

  Fig. 1 Chemical structure of natural products derived from Houttuynia cordata.

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  Fig. 2 Effect of aqueous or ethanolic extract of Houttuynia cordata on PMA-induced MUC5AC mucin production from NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of aqueous (A) or ethanolic (B) extract of Houttuynia cordata for 30 min and then stimulated with PMA (10 ng/mL) for 24 h. Cell lysates were collected for measurement of MUC5AC mucin production by ELISA. Each bar represents a mean ± S.E.M. of 3 culture wells in comparison with that of control set at 100%. Three independent experiments were performed and the representative data were shown. * significantly different from control (p<0.05). + significantly different from PMA alone (p<0.05). (cont: control, HC: Houttuynia cordata, concentration unit is µg/ mL.)

• 

  Fig. 3 Effect of quercitrin, quercetin or afzelin on PMA-induced MUC5AC mucin production from NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of quercitrin (A), quercetin (B) or afzelin (C) for 30 min and then stimulated with PMA (10 ng/mL) for 24 h. Cell lysates were collected for measurement of MUC5AC mucin production by ELISA. Three independent experiments were performed and the representative data were shown. Each bar represents a mean ± S.E.M. of three culture wells in comparison with that of control set at 100%. * significantly different from control (p<0.05). + significantly different from PMA alone (p<0.05). (cont: control, Q: quercetin, Qc: quercitrin, A: afzelin, concentration unit is µM.)

• 

  Fig. 4 Effect of quercitrin, quercetin or afzelin on PMA-induced MUC5AC mRNA expression in NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of quercitrin, quercetin or afzelin for 30 min and then stimulated with PMA (10 ng/mL) for 24 h. MUC5AC mRNA expression was measured by RT-PCR. Three independent experiments were performed and the representative data were shown. (cont: control, Q: quercetin, Qc: quercitrin, A: afzelin, concentration unit is µM.)

• 

  Fig. 5 Effect of quercitrin, quercetin or afzelin on EGF-induced MUC5AC mucin production from NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of quercitrin (A), quercetin (B) or afzelin (C) for 30 min and then stimulated with EGF (25 ng/mL) for 24 h. Cell lysates were collected for measurement of MUC5AC mucin production by ELISA. Three independent experiments were performed and the representative data were shown. Each bar represents a mean ± S.E.M. of three culture wells in comparison with that of control set at 100%. * significantly different from control (p<0.05). + significantly different from EGF alone (p<0.05). (cont: control, Q: quercetin, Qc: quercitrin, A: afzelin, concentration unit is µM.)

• 

  Fig. 6 Effect of quercitrin, quercetin or afzelin on EGF-induced MUC5AC mRNA expression in NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of quercitrin, quercetin or afzelin for 30 min and then stimulated with EGF (25 ng/mL) for 24 h. MUC5AC mRNA expression was measured by RT-PCR. Three independent experiments were performed and the representative data were shown. (cont: control, Q: quercetin, Qc: quercitrin, A: afzelin, concentration unit is µM.)

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