J Vet Sci.
2002 Dec;3(4):247-254.
Effects of Benzo[a]pyrene, 2-Bromopropane, Phenol and 2,3,7,8-Tetrachlorodibenzo-p-Dioxin on Proinflammatory Cytokines Gene Expression by Mice Spleen Cells
- Affiliations
-
- 1College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Korea. syryu@cnu.ac.kr
- 2College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea.
- 3College of Veterinary Medicine, Chonnam National University, Kwangju 500-757, Korea.
- 4Laboratory of Experimental Animal, Korea Cancer Center Hospital, Seoul 139-240, Korea.
- 5College of Veterinary Medicine, Texas A&M University, Tx 77843-4474, U.S.A.
Abstract
- The detrimental effects of environmental pollutants on the health of the individual are generally accepted, although the mechanisms of these effects remain to be incompletely understood. In the present study, we examined the effects of B[a]P, 2-BP, phenol and TCDD on proinflammatory cytokine gene expression in mice spleen cells which were stimulated with anti-CD3. 10-9M TCDD increased IFN gammar and TNF alpha gene expression, but suppressed IL-1 gene expression. 10-6M phenol inhibited IL-1, IL-6 and TNF alpha gene expression, and 10-6M of 2-BP downregulated TNF alpha gene expression. However, 10-6M of B[a]P did not influence on IL-1, IL-6, IFN gammar and TNF alpha gene expression. These findings suggest that TCDD may impair the immune functions of mice by enhancing proinflammatory cytokines production, whereas phenol and 2-BP may impair the functions by inhibiting the production of these cytokines.
Keyword
MeSH Terms
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Animals
Antigens, CD3/immunology
Apoptosis/drug effects
Benzo(a)pyrene/*toxicity
Cells, Cultured
Cytokines/*biosynthesis/genetics
Environmental Pollutants/*toxicity
Gene Expression/drug effects
Hydrocarbons, Brominated/*toxicity
Male
Mice
Mice, Inbred C3H
Phenol/*toxicity
RNA/chemistry/genetics
Reverse Transcriptase Polymerase Chain Reaction
Spleen/*drug effects/metabolism
Tetrachlorodibenzodioxin/*toxicity
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