Exp Mol Med.  2004 Jun;36(3):279-282.

Real-Time PCR analysis of af4 and dek genes expression in acute promyelocytic leukemiat (15;17)patients

Affiliations
  • 1Department of Medical Biology, Medical Faculty, University of Kocaeli, Kocaeli, Turkey. uozbek@istanbul.edu.tr
  • 2Department of Genetics, Institute for Experimental Medicine (DETAE), Istanbul University, Istanbul, Turkey.
  • 31st Department of Obstetrics and Gynecology, Semmelweis University, Budapest, Hungary.
  • 4Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey.
  • 5SSK Bakirkoy Hospital, Istanbul, Turkey.

Abstract

Among several newly identified oncogenes, dek and af4 are attractive targets for researchers interested with leukemia. In this study quantitative Real-Time RT-PCR technique was used to define alterations in expression of dek and af4 genes associated with acute promyelocytic leukaemia (APL) t (15; 17). RNA samples obtained from bone marrow aspirates of fourteen APL patients, cDNA portions were labelled with Syber Green 1 dye and LightCycler analysis have been performed. Expression changes in patients were found not significant in comparison to healthy donors for af4 (P=0.192) and dek (P= 0.0895). We suggest that af4 gene may have a role in leukomogenesis restricted to lymphoblastic lineage; also further studies must carry on with a larger series of patients in order to understand the relationship between the dek gene and APL. Our study was the first attempt for analysing dek and af4 genes in APL t (15; 17) patients by quantitative Real-Time RT-PCR. This rapid and sensitive method could be used to screen these genes in different types of leukaemia.

Keyword

af4; APL; dek; Gene expression; Real Time PCR
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