Exp Mol Med.  2004 Jun;36(3):279-282.

Real-Time PCR analysis of af4 and dek genes expression in acute promyelocytic leukemiat (15;17)patients

• Savli H
• Sirma S
• Nagy B
• Aktan M
• Dincol G
• Salcioglu
• Sarper N
• Ozbek U

Abstract

Among several newly identified oncogenes, dek and af4 are attractive targets for researchers interested with leukemia. In this study quantitative Real-Time RT-PCR technique was used to define alterations in expression of dek and af4 genes associated with acute promyelocytic leukaemia (APL) t (15; 17). RNA samples obtained from bone marrow aspirates of fourteen APL patients, cDNA portions were labelled with Syber Green 1 dye and LightCycler analysis have been performed. Expression changes in patients were found not significant in comparison to healthy donors for af4 (P=0.192) and dek (P= 0.0895). We suggest that af4 gene may have a role in leukomogenesis restricted to lymphoblastic lineage; also further studies must carry on with a larger series of patients in order to understand the relationship between the dek gene and APL. Our study was the first attempt for analysing dek and af4 genes in APL t (15; 17) patients by quantitative Real-Time RT-PCR. This rapid and sensitive method could be used to screen these genes in different types of leukaemia.

Keyword

af4; APL; dek; Gene expression; Real Time PCR

MeSH Terms

Adolescent
Adult
Child
Child, Preschool
Chromosomal Proteins, Non-Histone/*genetics/metabolism
Chromosomes, Human, Pair 15
Chromosomes, Human, Pair 17
DNA-Binding Proteins/*genetics/metabolism
Down-Regulation
Female
Gene Expression
Humans
Leukemia, Promyelocytic, Acute/*genetics/metabolism
Male
Middle Aged
Nuclear Proteins/*genetics/metabolism
Oncogene Proteins/*genetics/metabolism
Polymerase Chain Reaction
RNA, Messenger/analysis/metabolism
Translocation, Genetic
Up-Regulation