Exp Mol Med.  2003 Oct;35(5):454-459.

Real-time PCR analysis of the apoptosis related genes in ATRA treated APL t(15;17) patients

• Savli H
• Sirma S
• Nagy B
• Aktan M
• Dincol G
• Ozbek U

Abstract

All-trans retinoic acid (ATRA) treatment of the acute promyelocytic leukemia (APL) have subsequently resulted in cell apoptosis, but the molecular mechanism of this effect remains elusive. In order to understand a possible involvement of genes regulating apoptotic signal pathways, expression levels of bcl2, bax, dapk1, myc, bad, wt1, and mcl genes were analyzed during ATRA treatment in five APL patients with t (15;17) using Real- time PCR (LightCycler). Two samples from each patient were compared to each other: primary diagnostic sample and a sample taken at remission. Effect of the ATRA treatment was demonstrated by the concomitant induction of cd14 and il1beta genes in four patients. Also other apoptosis related genes were found down-regulated in general but especially the down regulated levels of wt1 and bax attract attention. Result suggested that ATRA dependent apoptosis of APL was under the control of both internal and external pathways without relationships to the amount of the blast populations. Ratio of bcl2 to bax may be more important for this regulation than the ratio of bcl2 to bad. Either bcl2 family or less known apoptosis related genes as wt1 will still be required to further studies in this setting.

MeSH Terms

Apoptosis/drug effects/*genetics
Chromosomes, Human, Pair 15/genetics
Chromosomes, Human, Pair 17/genetics
Gene Expression Profiling
Gene Expression Regulation, Neoplastic/*drug effects
HL-60 Cells
Human
Leukemia, Promyelocytic, Acute/*genetics
Polymerase Chain Reaction/*methods
Support, Non-U.S. Gov't
Time Factors
Translocation (Genetics)/*genetics
Tretinoin/*pharmacology