Exp Mol Med.  2001 Jun;33(2):59-63.

HNF1 and/or HNF3 may contribute to the tissue specific expression of glucokinase gene

• Cha JY
• Kim HI
• Im SS
• Li TZ
• Ahn YH

Abstract

A possible role of hepatocyte nuclear factor 1 (HNF1) or HNF3, a predominant trans-acting factors of hepatic or pancreatic beta-cells, was examined on the tissue specific interdependent expression of glucokinase (GK) in liver, H4IIE, HepG2, HIT-T15 and MIN6 cell line. The tissues or cell lines known to express GK showed abundant levels of HNF1 and HNF3 mRNA as observed in liver, H4IIE, HepG2, HIT-T15 and MIN6 cells, whereas they were not detected in brain, heart, NIH 3T3, HeLa cells. The promoter of glucokinase contains several HNF3 consensus sequences and are well conserved in human, mouse and rat. Transfection of the glucokinase promotor linked with luciferase reporter to liver or pancreatic beta cell lines showed high interacting activities with HNF1 and HNF3, whereas minimal activities were detected in the cells expressing very low levels of HNFs. The binding of HNF1 or HNF3 to the GK promoter genes was confirmed by electrophoretic mobility shift assay (EMSA). From these data, we propose that the expression of HNF1 and/or HNF3 may, in part, contribute to the tissue specific expression of GK.

Keyword

GK; GLUT2; HNF1; HNF3

MeSH Terms

3T3 Cells
Animal
Blotting, Northern
Cell Line
Cell Nucleus/metabolism
Cells, Cultured
DNA-Binding Proteins/genetics/*physiology
Genes, Reporter
Glucokinase/*biosynthesis/*genetics
Hela Cells
Human
Liver/metabolism
Luciferase/metabolism
Mice
Models, Genetic
Nuclear Proteins/genetics/*physiology
Plasmids/metabolism
Promoter Regions (Genetics)
Protein Binding
Rats
Support, Non-U.S. Gov't
Tissue Distribution
Transcription Factors/genetics/*physiology
Transcription, Genetic
Transfection