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Expression and Evaluation of Chikungunya Virus E1 and E2 Envelope Proteins for Serodiagnosis of Chikungunya Virus Infection

Cho B, Jeon BY, Kim J, Noh J, Kim J, Park M, Park S

PURPOSE: Chikungunya virus (CHIKV) causes endemic or epidemic outbreaks of CHIKV fever, which is a mosquitoe-transmitted viral disease in Africa, India, South-East Asia, and recently Southern Europe. Currently, serological diagnostic...
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Hepatitis C virus: virology and life cycle

Kim CW, Chang KM

Hepatitis C virus (HCV) is a positive sense, single-stranded RNA virus in the Flaviviridae family. It causes acute hepatitis with a high propensity for chronic infection. Chronic HCV infection can...
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Expression of Open Reading Frame 5 Protein of Porcine Reproductive and Respiratory Syndrome Virus Using Semliki Forest Virus Expression System

Jung HS, Hwang IW, Kim SM, Kim CJ, Shin KS, Kim HS

  • KMID: 1101569
  • J Vet Sci.
  • 2002 Mar;3(1):13-18.
The ORF5 gene encodes a major envelope glycoprotein (GP5), which is one of the three major proteins of porcine reproductive and respiratory syndrome virus (PRRSV). The GP5 protein has been...
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Immunogenicity of baculovirus expressed recombinant proteins of Japanese encephalitis virus in mice

Yang DK, Kweon CH, Kim BH, Lim SI, Kwon JH, Kim SH, Song JY, Han HR

  • KMID: 1114853
  • J Vet Sci.
  • 2005 Jun;6(2):125-133.
Genes encoding for the premembrane and envelope (prME), envelope (E) and nonstructural protein (NS1) of Japanese encephalitis virus (JEV) were cloned. Each protein was expressed in baculovirus expression system. Of...
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Importance of Specimen Type and Quality in Diagnosing Middle East Respiratory Syndrome

Huh HJ, Ko JH, Kim YE, Park CH, Hong G, Choi R, Yu S, Cho SY, Kang JM, Lee MK, Ki CS, Kang ES, Lee NY, Kim JW, Kim YJ, Ha YE, Kang CI, Chung DR, Peck KR, Song JH

No abstract available.
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The signal sequence of type II porcine reproductive and respiratory syndrome virus glycoprotein 3 is sufficient for endoplasmic reticulum retention

Kim DG, Song CS, Choi IS, Park SY, Lee JB, Lee SS

The glycoprotein 3 (GP3) of type II porcine reproductive and respiratory syndrome virus has the characteristic domains of a membrane protein. However, this protein has been reported to be retained...
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Recombinant Kluyveromyces lactis expressing highly pathogenic porcine reproductive and respiratory syndrome virus GP5 elicits mucosal and cell-mediated immune responses in mice

Zhao H, Wang Y, Ma Z, Wang Y, Feng WH

Currently, killed-virus and modified-live porcine reproductive and respiratory syndrome virus (PRRSV) vaccines are used to control porcine reproductive and respiratory syndrome. However, both types of vaccines have inherent drawbacks; accordingly,...
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From DCPD to NTCP: The long journey towards identifying a functional hepatitis B virus receptor

Li J, Tong S

Hepatitis B virus (HBV) is the prototype of hepatotropic DNA viruses (hepadnaviruses) infecting a wide range of human and non-human hosts. Previous studies with duck hepatitis B virus (DHBV) identified...
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Molecular characterization of duck enteritis virus CHv strain UL49.5 protein and its colocalization with glycoprotein M

Lin M, Jia R, Wang M, Gao X, Zhu D, Chen S, Liu M, Yin Z, Wang Y, Chen X, Cheng A

The UL49.5 gene of most herpesviruses is conserved and encodes glycoprotein N. However, the UL49.5 protein of duck enteritis virus (DEV) (pUL49.5) has not been reported. In the current study,...
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Agar gel immunodiffusion analysis using baculovirus-expressed recombinant bovine leukemia virus envelope glycoprotein (gp51/gp30T-)

Lim SI, Jeong W, Tark DS, Yang DK, Kweon CH

Bovine leukemia virus (BLV) envelope glycoprotein (gp51/gp30T-), consisting of BLV gp51 and BLV gp30 that lacked its C-terminal transmembrane domain, was expressed in insect cells under the control of the...
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Little role of anti-gB antibodies in neutralizing activity of patient's sera with human cytomegalovirus (HCMV) infection

Park JW, Kim DJ, Kim J, Park CG, Hwang ES, Cha CY

Human cytomegalovirus (HCMV) gB is known to play important roles in cell surface attachment, virion penetration, spread of infection from cell to cell, and provocation of neutralizing antibody. This study...
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Phylogenetic characterization of genes encoding for glycoprotein 5 and membrane protein of PRRSV isolate HH08

Wang M, Li G, Yin J, Ren X

A porcine reproductive and respiratory syndrome virus (PRRSV) was obtained from clinic samples. Genes 5 and 6 encoding for the viral glycoprotein 5 and a membrane protein of the PRRSV...
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Positive effects of porcine IL-2 and IL-4 on virus-specific immune responses induced by the porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 DNA vaccine in swine

Tang D, Liu J, Li C, Zhang H, Ma P, Luo X, Zeng Z, Hong N, Liu X, Wang B, Wang F, Gan Z, Hao F

The purpose of this study was to investigate the effects of porcine interleukin (IL)-2 and IL-4 genes on enhancing the immunogenicity of a porcine reproductive and respiratory syndrome virus ORF5...
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Analytical and Clinical Validation of Six Commercial Middle East Respiratory Syndrome Coronavirus RNA Detection Kits Based on Real-Time Reverse-Transcription PCR

Kim MN, Ko YJ, Seong MW, Kim JS, Shin BM, Sung H

BACKGROUND: During the 2015 outbreak of Middle East Respiratory Syndrome coronavirus (MERS-CoV), six different commercial MERS-CoV RNA detection kits based on real-time reverse-transcription polymerase chain reaction (rRT-PCR) were available in...
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Viral loads and E2/NS1 region sequences of hepatitis C virus in hepatocellular carcinoma and surrounding liver

Park CK, Park TR, Kim YB, Kim HY, Yoo JY, Kim CH, Choo SH, Cho JM

  • KMID: 1038874
  • Korean J Intern Med.
  • 1997 Jan;12(1):28-33.
OBJECTIVES: Numerous epidemiologic data have documented that chronic infection by hepatitis C virus (HCV) is a major risk factor for the development of hepatocellular carcinoma (HCC). But the molecular mechanism...
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Molecular characterization of full-length genome of Japanese encephalitis virus (KV1899) isolated from pigs in Korea

Yang DK, Kim BH, Kweon CH, Kwon JH, Lim SI, Han HR

  • KMID: 1099832
  • J Vet Sci.
  • 2004 Sep;5(3):197-205.
We have determined the complete nucleotide and deduced amino acid sequences of the Japanese encephalitis virus (JEV) strain KV1899, isolated from a fattening pig in Korea. In comparison with 22...
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Comparative study of synonymous codon usage variations between the nucleocapsid and spike genes of coronavirus, and C-type lectin domain genes of human and mouse

Ahn I, Jeong BJ, Son HS

Coronaviruses (CoVs) are single-stranded RNA viruses which contain the largest RNA genomes, and severe acute respiratory syndrome coronavirus (SARS-CoV), a newly found group 2 CoV, emerged as infectious disease with...
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Modulation of protective immunity against herpes simplex virus via mucosal genetic co-transfer of DNA vaccine with beta2-adrenergic agonist

Kim SB, Han YW, Rahman MM, Kim SJ, Yoo DJ, Kang SH, Kim K, Eo SK

Cholera toxin, which has been frequently used as mucosal adjuvant, leads to an irreversible activation of adenylyl cyclase, thereby accumulating cAMP in target cells. Here, it was assumed that beta2-adrenergic...
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Distribution of hepatitis B virus genotypes in Korea

Cho JH, Yoon KH, Lee KE, Park DS, Lee YJ, Moon HB, Lee KR, Choi CS, Cho EY, Kim HC

BACKGROUND/AIMS: Considering the incidence of prevailing hepatitis B virus (HBV) genotypes in neighboring nations, the predominance of genotype C in Korea is exceptional and needs to be confirmed by nationwide...
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Prevalence of tick-borne encephalitis virus in ticks from southern Korea

Ko S, Kang JG, Kim SY, Kim HC, Klein TA, Chong ST, Sames WJ, Yun SM, Ju YR, Chae JS

The prevalence of tick-borne encephalitis virus (TBEV) in southern Korea was determined by collecting ticks using tick drags. A total of 4,077 of 6,788 ticks collected were pooled (649 pools)...
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