J Korean Med Sci.  2000 Apr;15(2):133-138. 10.3346/jkms.2000.15.2.133.

Little role of anti-gB antibodies in neutralizing activity of patient's sera with human cytomegalovirus (HCMV) infection

• Park JW
• Kim DJ
• Kim J
• Park CG
• Hwang ES
• Cha CY

• KMID: 648113
• DOI: http://doi.org/10.3346/jkms.2000.15.2.133

Abstract

Human cytomegalovirus (HCMV) gB is known to play important roles in cell surface attachment, virion penetration, spread of infection from cell to cell, and provocation of neutralizing antibody. This study was performed to determine the role of anti-HCMV gB antibody in overall neutralizing response in patients with HCMV infection and healthy control with past infection. HCMV gB was stably expressed in 293 cells. With the stable cell line expressing gB as a specific immunosorbent, anti-gB antibody was removed from the current and past HCMV-infected sera and the remaining neutralizing activity was measured by plaque assay. It was shown that 19-50% of the total virus-neutralizing activity of sera with past HCMV infections was derived from anti-gB antibody, but anti-gB antibody had little effect on the total serum virus-neutralizing activity in patients currently infected with HCMV. This result suggests that neutralizing antibody to HCMV gB may reflect disease status.

Keyword

Cytomegalovirus; Antibodies, Viral; Neutralization Tests; Immunosorbents

MeSH Terms

Adult
Antibodies, Monoclonal
Antibodies, Viral/immunology*
Antibodies, Viral/blood
Antigens, Viral/immunology
Antigens, Viral/genetics
Cells, Cultured
Cytomegalovirus/immunology*
Cytomegalovirus Infections/prevention & control
Cytomegalovirus Infections/immunology*
Female
Fetus/cytology
Fibroblasts/cytology
Gene Expression Regulation, Viral/immunology
Human
Immunosorbents
Lung/cytology
Male
Middle Age
Neutralization Tests
Recombinant Proteins/genetics
Viral Envelope Proteins/immunology*
Viral Vaccines