J Vet Sci.
2010 Sep;11(3):197-203. 10.4142/jvs.2010.11.3.197.
Prevalence of tick-borne encephalitis virus in ticks from southern Korea
- Affiliations
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- 1Veterinary Internal Medicine, Research Institute and BK21 Program for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. jschae@snu.ac.kr
- 2Division of Arboviruses, Center for Immunology and Pathology, Korea National Institute of Health, Seoul 122-701, Korea. juyran@nih.go.kr
- 35th Medical Detachment, 168th Multifunctional Medical Battalion, 65th Medical Brigade, Unit #15247, APO AP 96205-5247, Seoul, Korea.
- 4Force Health Protection and Preventive Medicine, 65th Medical Brigade, Unit #15281, APO AP 96205-5281, Seoul, Korea.
- 5Defense Logistics Agency, 8725 John J. Kingman Road, Suite 2639 Attn: DES-EQ, Fort Belvoir, VA 22060-6221, USA.
- KMID: 1093493
- DOI: http://doi.org/10.4142/jvs.2010.11.3.197
Abstract
- The prevalence of tick-borne encephalitis virus (TBEV) in southern Korea was determined by collecting ticks using tick drags. A total of 4,077 of 6,788 ticks collected were pooled (649 pools) according to collection site, species, and developmental stage and assayed for TBEV. The TBEV protein E and NS5 gene fragments were detected using RT-nested PCR in six pools of nymphs collected from Jeju Island (2,491 ticks). The minimum field detection rates for TBEV were 0.17% and 0.14% for Haemaphysalis longicornis and Haemayphysalis. flava nymphs, respectively. The 252 bp NS5 and 477 bp protein E gene amplicons were sequenced. Phylogenetic analysis showed that the NS5 and protein E genes of the Jeju strain were clustered with Western subtype (98.0% and 99.4% identity, respectively). The Western subtype of TBEV is endemic in Korea, including Jeju Island. The study of vector and zoonotic host susceptibility to TBEV is required to better understand its potential impact on public health.
MeSH Terms
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Animals
Arachnid Vectors/*virology
Base Sequence
DNA Primers/genetics
Encephalitis Viruses, Tick-Borne/classification/*genetics
Encephalitis, Tick-Borne/*epidemiology
Molecular Sequence Data
*Phylogeny
Prevalence
Republic of Korea/epidemiology
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Ticks/*virology
Viral Envelope Proteins/genetics
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