- Plasmid profiling of Flavobacterium psychrophilum isolates from ayu (Plecoglossus altivelis altivelis) and other fish species in Japan
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Kim JH, Gomez DK, Nakai T, Park SC
- KMID: 1106162
- J Vet Sci.
- 2010 Mar;11(1):85-87.
- doi: 10.4142/jvs.2010.11.1.85
In order to evaluate the genetic variability of the causative agent of cold water disease (CWD), plasmid profiling was used to characterize Flavobacterium (F.) psychrophilum isolates (n = 169). Size... -
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- Enhanced immune response with foot and mouth disease virus VP1 and interleukin-1 fusion genes
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Park JH, Kim SJ, Oem JK, Lee KN, Kim YJ, Kye SJ, Park JY, Joo YS
- KMID: 1089910
- J Vet Sci.
- 2006 Sep;7(3):257-262.
- doi: 10.4142/jvs.2006.7.3.257
The capsid of the foot and mouth disease (FMD) virus carries the epitopes that are critical for inducing the immune response. In an attempt to enhance the specific immune response,... -
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- Improved expression by cytomegalovirus promoter/enhancer and behavior of vascular endothelial growth factor gene after myocardial injection of naked DNA
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Jeong JO, Byun JH, Jeon ES, Gwon HC, Lim YS, Park JW, Yeo SJ, Lee YJ, Kim SY, Kim DK
- KMID: 931878
- Exp Mol Med.
- 2002 Sep;34(4):278-284.
Direct injection of the vascular endothelial growth factor (VEGF) gene plasmid DNA into the myocardium was shown to induce development of new blood vessels to increase the circulation in... -
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- p53 overexpression represses androgen-mediated induction of NKX3.1 in a prostate cancer cell line
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Jiang A, Yu C, Zhang P, Chen W, Liu W, Hu X, Zhang J
- KMID: 1115940
- Exp Mol Med.
- 2006 Dec;38(6):625-633.
Prostate cancer is a disease involving complicated multiple-gene alterations. Both NKX3.1 and p53 are related to prostate cancer and play crucial roles in prostate cancer progression. However, little is known... -
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- Optimization of Streptomyces bacteriophage phiC31 integrase system to prevent post integrative gene silencing in pulmonary type II cells
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Aneja MK, Geiger J, Imker R, Uzgun S, Kormann M, Hasenpusch G, Maucksch C, Rudolph C
- KMID: 1099868
- Exp Mol Med.
- 2009 Dec;41(12):919-934.
- doi: 10.3858/emm.2009.41.12.098
phiC31 integrase has emerged as a potent tool for achieving long-term gene expression in different tissues. The present study aimed at optimizing elements of phiC31 integrase system for alveolar type... -
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- Expression of Open Reading Frame 5 Protein of Porcine Reproductive and Respiratory Syndrome Virus Using Semliki Forest Virus Expression System
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Jung HS, Hwang IW, Kim SM, Kim CJ, Shin KS, Kim HS
- KMID: 1101569
- J Vet Sci.
- 2002 Mar;3(1):13-18.
The ORF5 gene encodes a major envelope glycoprotein (GP5), which is one of the three major proteins of porcine reproductive and respiratory syndrome virus (PRRSV). The GP5 protein has been... -
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- Prediction of Putative Resistance Islands in a Carbapenem-Resistant Acinetobacter baumannii Global Clone 2 Clinical Isolate
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Lee Y, D'Souza R, Yong D, Lee K
- KMID: 2373551
- Ann Lab Med.
- 2016 Jul;36(4):320-324.
- doi: 10.3343/alm.2016.36.4.320
BACKGROUND: We investigated the whole genome sequence (WGS) of a carbapenem-resistant Acinetobacter baumannii isolate belonging to the global clone 2 (GC2) and predicted resistance islands using a software tool. METHODS: A.... -
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- The signal sequence of type II porcine reproductive and respiratory syndrome virus glycoprotein 3 is sufficient for endoplasmic reticulum retention
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Kim DG, Song CS, Choi IS, Park SY, Lee JB, Lee SS
- KMID: 1705561
- J Vet Sci.
- 2013 Sep;14(3):307-313.
- doi: 10.4142/jvs.2013.14.3.307
The glycoprotein 3 (GP3) of type II porcine reproductive and respiratory syndrome virus has the characteristic domains of a membrane protein. However, this protein has been reported to be retained... -
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- Characterization of a brain tumor cell line established from transgenic mice expressing the vasopressin SV-40 T antigen
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Kim SH, Kim MO, Lee SR, Kim KS, Lee TH, Lee HT, Ha JH, Kim TY, Ryoo ZY
- KMID: 1094144
- Exp Mol Med.
- 2006 Jun;38(3):196-202.
We previously reported that transgenic mice produced with a transgene consisting of the SV40 T antigen and vasopressin without the 3'-flanking region exhibit brain tumors and lymphoma. In this study,... -
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- Plasmid-encoded AmpC beta-lactamases: how far have we gone 10 years after the discovery?
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Bauernfeind A, Chong Y, Lee K
- KMID: 711593
- Yonsei Med J.
- 1998 Dec;39(6):520-525.
- doi: 10.3349/ymj.1998.39.6.520
The dogma that ampC genes are located exclusively on the chromosome was dominant until about 10 years ago. Since 1989 over 15 different plasmid-encoded AmpC beta-lactamases have been reported from... -
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- Co-Immunization of Plasmid DNA Encoding IL-12 and IL-18 with Bacillus Calmette-Guerin Vaccine against Progressive Tuberculosis
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Jeon BY, Eoh H, Ha SJ, Bang H, Kim SC, Sung YC, Cho SN
- KMID: 1058823
- Yonsei Med J.
- 2011 Nov;52(6):1008-1015.
- doi: 10.3349/ymj.2011.52.6.1008
PURPOSE: Bacillus Calmette-Guerin (BCG) vaccine has widely been used to immunize against tuberculosis, but its protective efficacy is variable in adult pulmonary tuberculosis, while it is not efficiently protective against... -
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- Emerging antimicrobial resistance, plasmid profile and pulsed-field gel electrophoresis pattern of the endonuclease-digested genomic DNA of Neisseria gonorrhoeae
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Lee K, Shin JW, Lim JB, Kim YA, Yong D, Oh HB, Chong Y
- KMID: 711761
- Yonsei Med J.
- 2000 Jun;41(3):381-386.
- doi: 10.3349/ymj.2000.41.3.381
Resistant gonococci are very prevalent in many countries, particularly in Asia. This study was conducted to determine the trend of resistance, the effect of decreasing the ciprofloxacin susceptibilities of gonococci... -
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- Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice
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Jung BG, Cho SJ, Ko JH, Lee BJ
- KMID: 1093495
- J Vet Sci.
- 2010 Sep;11(3):213-220.
- doi: 10.4142/jvs.2010.11.3.213
Interleukin (IL)-10 exerts potent anti-inflammatory effects by suppression of both T-help (Th) 1 and Th2 cells. Previous studies have reported that IL-10 can ameliorate various inflammatory disorders. The present study... -
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- Silencing IL-23 expression by a small hairpin RNA protects against asthma in mice
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Li Y, Sun M, Cheng H, Li S, Liu L, Qiao H, Hua S, Lu J
- KMID: 1104389
- Exp Mol Med.
- 2011 Apr;43(4):197-204.
- doi: 10.3858/emm.2011.43.4.024
To determine the impact of IL-23 knockdown by RNA interference on the development and severity of ovalbumin (OVA)-induced asthmatic inflammation, and the potential mechanisms in mice, the IL-23-specific RNAi-expressing pSRZsi-IL-23p19... -
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- Prevalence of Plasmid-mediated Quinolone Resistance and Its Association with Extended-spectrum Beta-lactamase and AmpC Beta-lactamase in Enterobacteriaceae
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Jeong HS, Bae IK, Shin JH, Jung HJ, Kim SH, Lee JY, Oh SH, Kim HR, Chang CL, Kho WG, Lee JN
- KMID: 1781689
- Korean J Lab Med.
- 2011 Oct;31(4):257-264.
- doi: 10.3343/kjlm.2011.31.4.257
BACKGROUND: We investigated the prevalence of plasmid-mediated quinolone resistance and its association with extended-spectrum beta-lactamase (ESBL) and AmpC beta-lactamase in Enterobacteriaceae. METHODS: A total of 347 non-duplicated isolates of Enterobacteriaceae were... -
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- Application of HTB-SiHa Cells Transfected with a Recombinant Plasmid for External Quality Assessment of Chlamydia trachomatis PCR
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Zhang K, Huo H, Sun Y, Wang L, Zhang R, Lin G, Xie J, Wang Q, Li J
- KMID: 1791950
- Ann Lab Med.
- 2014 Sep;34(5):360-366.
- doi: 10.3343/alm.2014.34.5.360
BACKGROUND: The participation of laboratories in external quality assessment (EQA) programs is required for the quality assurance of nucleic acid amplification of Chlamydia trachomatis. This study aimed to construct a... -
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- Improved cycle sequencing of GC-rich DNA template
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Choi JS, Kim JS, Joe CO, Kim SH, Ha KS, Park YM
- KMID: 755022
- Exp Mol Med.
- 1999 Mar;31(1):20-24.
Even when DNA sequencing of purified DNA template failed under the optimal condition, it can be generally contributed to high GC content. GC-rich region of template causes a secondary structure to produce... -
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- Synergistic effect of peroxiredoxin II antisense on cisplatin-induced cell death
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Yoo YD, Chung YM, Park JK, Ahn CM, Kim SK, Kim HJ
- KMID: 931877
- Exp Mol Med.
- 2002 Sep;34(4):273-277.
Peroxiredoxin II (Prx II) is known not only to protect cells from oxidative damage caused by hydrogen peroxide (H2O2), but also to endow cancer cells with resistance to both... -
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- Optimal salt concentration of vehicle for plasmid DNA enhances gene transfer mediated by electroporation
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Lee MJ, Cho SS, Jang HS, Lim YS, You JR, Park JW, Suh HR, Kim JA, Park JS, Kim DK
- KMID: 931876
- Exp Mol Med.
- 2002 Sep;34(4):265-272.
In vivo electroporation has emerged as a leading technology for developing nonviral gene therapies, and the various technical parameters governing electroporation efficiency have been optimized by both theoretical and... -
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