J Vet Sci.  2010 Mar;11(1):85-87. 10.4142/jvs.2010.11.1.85.

Plasmid profiling of Flavobacterium psychrophilum isolates from ayu (Plecoglossus altivelis altivelis) and other fish species in Japan

• Kim JH
• Gomez DK
• Nakai T
• Park SC

• KMID: 1106162
• DOI: http://doi.org/10.4142/jvs.2010.11.1.85

Abstract

In order to evaluate the genetic variability of the causative agent of cold water disease (CWD), plasmid profiling was used to characterize Flavobacterium (F.) psychrophilum isolates (n = 169). Size analysis of plasmids in F. psychrophilum isolates (n = 128) from several fish species demonstrated that six kinds of plasmids were harbored, and ayu isolates had different profiles compared to other isolates. Moreover, multiple isolates (n = 41) from CWD outbreaks in 2002 to 2003 at a single ayu farm were examined to determine differences between isolates from successive outbreaks and showed different profiles by the sources of seedlings.

Keyword

ayu; CWD; Flavobacterium psychrophilum; plasmid profile

MeSH Terms

Animals
DNA, Bacterial/genetics
Disease Outbreaks/*veterinary
Electrophoresis, Agar Gel/veterinary
Fish Diseases/genetics/*microbiology
Flavobacteriaceae Infections/microbiology/*veterinary
Flavobacterium/genetics/*isolation & purification
Genetic Variation/*genetics
Japan
*Osmeriformes
Plasmids/genetics

Figure

• 

  Fig. 1 Plasmids profiles extracted from Flavobacterium psychrophilum isolates using 0.70% agarose ME-TAE gels. Lane M; marker (λ DNA-Hind III digest), Lane 1; 3.5 and 2.4 kb (ayu isolate), Lane 2; 3.5 and 2.4 kb (crucian carp isolate), Lane 3; 3.5 and 1.8 kb (ayu isolate), Lane 4; 3.5 kb (ayu isolate), Lane 5; 2.7 kb (rainbow trout isolate), Lane 6; 2.4 kb (ayu isolate), Lane 7; 1.8 kb (ayu isolate), Lane 8; 100 and 23 kb (crucian carp isolate), Lane 9; none (ayu isolate).

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