Exp Mol Med.
2002 Sep;34(4):273-277.
Synergistic effect of peroxiredoxin II antisense on cisplatin-induced cell death
- Affiliations
-
- 1Korea University Cancer Institute, Korea University College of Medicine, Seoul, Korea. khj57@yumc.yonsei.ac.kr
- 2Genomic Research Center for Lung and Breast/Ovarian Cancers, Korea University College of Medicine, Seoul, Korea.
- 3Brain Korea 21 Biomedical Sciences, Korea University College of Medicine, Seoul, Korea.
- 4Department of Life Science, Sogang University, Seoul, Korea.
- 5Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
Abstract
- Peroxiredoxin II (Prx II) is known not only to protect cells from oxidative damage caused by hydrogen peroxide (H2O2), but also to endow cancer cells with resistance to both H2O2 and cisplatin and to grant them radioresistance. In this study, we examined whether Prx II antisense could enhance cisplatin-induced cell death. When gastric cancer cells were transfected with various concentrations of Prx II antisense plasmid, pPrxII/AS, and then treated with the same concentrations of cisplatin, Prx II antisense enhanced cisplatin-induced cell death. The combination index (CI) at all doses of the combination was below 1, indicating that Prx II antisense sensitized cisplatin-induced cell death. This synergism was also observed in the cells transfected with a Prx II antisense oligomer. Our present results, therefore, suggest that Prx II antisense would be a very good sensitizer for cisplatin, and that Prx II as a target for chemosensitizers constitutes a promising avenue for future research.
Keyword
MeSH Terms
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Antineoplastic Agents/*pharmacology
Antioxidants/metabolism
Apoptosis/drug effects
Cell Death/*drug effects
Cisplatin/*pharmacology
Dose-Response Relationship, Drug
Drug Synergism
Genetic Vectors
Human
Oligonucleotides, Antisense/*metabolism
Peroxidases/*metabolism
Plasmids/genetics/metabolism
Stomach Neoplasms/*metabolism
Tumor Cells, Cultured
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