Exp Mol Med.
2006 Jun;38(3):196-202.
Characterization of a brain tumor cell line established from transgenic mice expressing the vasopressin SV-40 T antigen
- Affiliations
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- 1School of Life Science and Biotechnology, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea. jaewoong64@hanmail.net
- 2Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea.
- 3Department of Animal Science, Graduate School of Konkuk University, Seoul 143-701, Korea.
- 4Department of Oral Biochemistry, College of Dentistry, Chonnam National University, Gwangju 500-757, Korea.
- 5Department of Immunobiology and Dermatology, College of Medicine, Catholic University, Seoul 137-040, Korea.
Abstract
- We previously reported that transgenic mice produced with a transgene consisting of the SV40 T antigen and vasopressin without the 3'-flanking region exhibit brain tumors and lymphoma. In this study, transgenic mice were produced with the fusion gene containing the SV40 T antigen and the whole vasopressin gene with the 3'-flanking region. Six transgenic mice were generated, five which died after 2-6 weeks. The remaining founder mouse was investigated for fusion gene expression and tumor progression at the age of 6 weeks. Brain tumor cells were characterized for phenotypes and transgene expression. During in vitro cell cultures, the phenotypic appearances at 10, 20, and 30 passages were as a uniform monolayer with similar growth rates. The site of SV40 T antigen integration was in the A2 region of chromosome 11, and SV40 T antigen was expressed at the same level in cells of both earlier and later passages. Thirty passages were probably insufficient to reach crisis and immortalization. These cells enriched brain tumor cell compositions with astrocytes and neuronal cells.
MeSH Terms
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Vasopressins/genetics/*metabolism
Transgenes/genetics
Recombinant Fusion Proteins/genetics/metabolism
Plasmids/genetics
Mice, Transgenic
Mice, Inbred ICR
Mice
In Situ Hybridization, Fluorescence/methods
Immunoenzyme Techniques
Gene Expression/genetics
Cell Proliferation
Cell Line, Tumor
Brain Neoplasms/genetics/*metabolism/pathology
Blotting, Western
Antigens, Polyomavirus Transforming/genetics/*metabolism
Animals
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