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Comparison of Diagnostic Performance of Three Real-Time PCR Kits for Detecting Mycobacterium Species

Cho SY, Kim MJ, Suh JT, Lee HJ

PURPOSE: PCR is widely used for rapidly and accurately detecting Mycobacterium Species. The purpose of this study was to assess the diagnostic performance of three real-time PCR kits and evaluate...
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Hepatic tuberculosis presenting as a large liver mass

Shin YM

No abstract available.
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Lung Infection Caused by Mycobacterium riyadhense Confused with Mycobacterium tuberculosis: The First Case in Korea

Choi JI, Lim JH, Kim SR, Lee SH, Park JS, Seo KW, Jeon JB, Jeong J

A slowly growing, non-chromogenic mycobacterial strain was isolated from sputum and bronchial lavage fluid samples of a patient presenting with productive cough, blood-tinged sputum, low-grade fever, and weakness. A positive...
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Molecular Strain Typing of Mycobacterium tuberculosis: a Review of Frequently Used Methods

Ei PW, Aung WW, Lee JS, Choi GE, Chang CL

Tuberculosis, caused by the bacterium Mycobacterium tuberculosis, remains one of the most serious global health problems. Molecular typing of M. tuberculosis has been used for various epidemiologic purposes as well...
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Isoniazid resistance and the point mutation of codon 463 of katG gene of Mycobacterium tuberculosis

Shim TS, Yoo CG, Han SK, Shim YS, Kim YW

It has long been known that almost all isoniazid (INH) resistant mycobacteria lose the catalase and peroxidase activities along with reduced or no virulence for guinea pigs. Recently resistance...
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Detection of Mycobacterium tuberculosis in clinical samples from patients with tuberculosis or other pulmonary diseases by polymerase chain reaction

Yoon KH, Cho SN, Lee TY, Cheon SH, Chang J, Kim SK, Chong Y, Chung DH, Lee WY, Kim JD

Polymerase chain reaction (PCR) using primers targeting the IS6110 repetitive sequence was employed to detect Mycobacterium tuberculosis in 228 samples from patients with tuberculosis or other pulmonary diseases and controls,...
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Current Issues on Molecular and Immunological Diagnosis of Tuberculosis

Cho SN

aboratory diagnosis of tuberculosis (TB) traditionally relies on smear microscopy and culture of Mycobacterium tuberculosis from clinical samples. With recent advances in technology, there have been numerous efforts to develop...
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A Case of Gallbladder Tuberculosis Diagnosed by Positive Tuberculosis-Polymerase Chain Reaction

Ryu MJ, Jeon TJ, Park JY, Choi Y, Baek SS, Sinn DH, Oh TH, Kim JY

Gallbladder tuberculosis is an extremely rare disease that is rarely reported in the literature. Arriving at the correct diagnosis of gallbladder tuberculosis is difficult, and it is usually made by...
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Comparative Evaluation of Several Gene Targets for Designing a Multiplex-PCR for an Early Diagnosis of Extrapulmonary Tuberculosis

Raj A, Singh N, Gupta KB, Chaudhary , Yadav A, Chaudhary , Agarwal K, Varma-Basil M, Prasad R, Khuller GK, Mehta PK

PURPOSE: Diagnosis of extrapulmonary tuberculosis (EPTB) poses serious challenges. A careful selection of appropriate gene targets is essential for designing a multiplex-polymerase chain reaction (M-PCR) assay. MATERIALS AND METHODS: We compared...
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Assessment of the Quantitative Ability of AdvanSure TB/NTM Real-Time PCR in Respiratory Specimens by Comparison with Phenotypic Methods

Lee H, Park KG, Lee G, Park J, Park YG, Park YJ

Accurate quantification of mycobacterial load is important to evaluate disease severity and to monitor the course of treatment in tuberculosis (TB). We evaluated the quantitative capability of the AdvanSure TB/NTM...
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Evaluation of Propidium Monoazide Real-Time PCR for Early Detection of Viable Mycobacterium tuberculosis in Clinical Respiratory Specimens

Kim YJ, Lee SM, Park BK, Kim SS, Yi J, Kim HH, Lee EY, Chang CL

BACKGROUND: Conventional acid-fast bacilli (AFB) staining cannot differentiate viable from dead cells. Propidium monoazide (PMA) is a photoreactive DNA-binding dye that inhibits PCR amplification by DNA modification. We evaluated whether...
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Evaluation of the Performances of AdvanSure TB/NTM Real Time PCR Kit for Detection of Mycobacteria in Respiratory Specimens

Kim YJ, Park MY, Kim SY, Cho SA, Hwang SH, Kim HH, Lee EY, Jeong J, Kim KH, Chang CL

BACKGROUND: PCR is a widely used method for rapid and accurate diagnosis of mycobacteriosis. The sensitivity and specificity of a real time PCR kit newly developed in Korea were evaluated...
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A Case of Tuberculous Liver Abscess Developed during Chemotherapy for Tuberculous Peritonitis as Paradoxical Response

Kim TK, Choi CW, Ha JK, Jang HH, Park SB, Kim HW, Kang DH

Tuberculous liver abscesses are rare. Paradoxical response in tuberculosis is common and occurred between 2 weeks and 12 weeks after anti-tuberculous medication. We report here a case of tuberculous liver...
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