- Transduction of eGFP Gene to Human Embryonic Stem Cells and Their Characterization
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Kim YY, Ku SY, Park YB, Oh SK, Moon SY, Choi YM
- KMID: 2288259
- Korean J Reprod Med.
- 2009 Dec;36(4):283-292.
OBJECTIVE: Human embryonic stem cells (hESCs) can proliferate indefinitely and differentiate into all kinds of cell types in vitro. Therefore, hESCs can be used as a cell source for cell-based... -
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- Use of In Vivo and In Vitro Systems to Select Leishmania amazonensis Expressing Green Fluorescent Protein
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Costa SD, de Assis Golim M, Rossi-Bergmann B, Costa FT, Giorgio S
- KMID: 1715740
- Korean J Parasitol.
- 2011 Dec;49(4):357-364.
- doi: 10.3347/kjp.2011.49.4.357
Various Leishmania species were engineered with green fluorescent protein (GFP) using episomal vectors that encoded an antibiotic resistance gene, such as aminoglycoside geneticin sulphate (G418). Most reports of GFP-Leishmania have... -
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- Mouse Fyn induces pseudopodium formation in Chinese hamster ovary cells
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An L, Liu S, Zhang W, Zhang Y, Huang Y, Hu X, Chen S, Zhao S
- KMID: 1737616
- J Vet Sci.
- 2014 Mar;15(1):111-115.
- doi: 10.4142/jvs.2014.15.1.111
Molecular mechanisms underlying the effects of Fyn on cell morphology, pseudopodium movement, and cell migration were investigated. The Fyn gene was subcloned into pEGFP-N1 to produce pEGFP-N1-Fyn. Chinese hamster ovary... -
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- In Vitro Infectivity Assessment by Drug Susceptibility Comparison of Recombinant Leishmania major Expressing Enhanced Green Fluorescent Protein or EGFP-Luciferase Fused Genes with Wild-Type Parasite
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Sadeghi S, Seyed N, Etemadzadeh MH, Abediankenari S, Rafati S, Taheri T
- KMID: 2381755
- Korean J Parasitol.
- 2015 Aug;53(4):385-394.
- doi: 10.3347/kjp.2015.53.4.385
Leishmaniasis is a worldwide uncontrolled parasitic disease due to the lack of effective drug and vaccine. To speed up effective drug development, we need powerful methods to rapidly assess drug... -
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- Recombinant AAV Vector with MITF-M Promoter for Melanoma Gene Therapy
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Park SW, Paik SY
- KMID: 1434773
- J Bacteriol Virol.
- 2012 Mar;42(1):57-62.
- doi: 10.4167/jbv.2012.42.1.57
We have developed the recombinant adeno-associated virus (AAV) carrying the EGFP gene under the control of the microphtalmia-associated transcription factor-M (MITF-M) promoter region for melanoma-specific expression. MITF-M distal enhancer (MDE)... -
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- Organotypic slice culture of the hypothalamic paraventricular nucleus of rat
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Cho ES, Lee SY, Park JY, Hong SG, Ryu PD
- KMID: 1104231
- J Vet Sci.
- 2007 Mar;8(1):15-20.
- doi: 10.4142/jvs.2007.8.1.15
Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged... -
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- microRNA-183 is Essential for Hair Cell Regeneration after Neomycin Injury in Zebrafish
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Kim CW, Han JH, Wu L, Choi JY
- KMID: 2418861
- Yonsei Med J.
- 2018 Jan;59(1):141-147.
- doi: 10.3349/ymj.2018.59.1.141
PURPOSE: microRNAs (miRNAs) are non-coding RNAs composed of 20 to 22 nucleotides that regulate development and differentiation in various organs by silencing specific RNAs and regulating gene expression. In the... -
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- Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model
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He XY, Ma LB, He XN, Si WT, Zheng YM
- KMID: 2413163
- J Vet Sci.
- 2016 Jun;17(2):145-152.
- doi: 10.4142/jvs.2016.17.2.145
Previous studies have established a bovine mammary gland epithelia cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target... -
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- Promoter effects of adeno-associated viral vector for transgene expression in the cochlea in vivo
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Liu Y, Okada T, Nomoto T, Ke X, Kume A, Ozawa K, Xiao S
- KMID: 1094657
- Exp Mol Med.
- 2007 Apr;39(2):170-175.
The aims of this study were to evaluate the expression of enhanced green fluorescent protein (EGFP) driven by 6 different promoters, including cytomegalovirus IE enhancer and chicken beta-actin promoter (CAG),... -
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- Sec13 induces genomic instability in U2OS cells
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Sihn CR, Suh EJ, Lee KH, Kim SH
- KMID: 1056239
- Exp Mol Med.
- 2005 Jun;37(3):255-260.
Sec13p has been known as an endoplasmic reticulum-Golgi transport protein. Recently, it has also been shown to be required for the formation of septation in the fission yeast Schizosaccharomyces pombe.... -
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- Engraftment of Intraperitoneally Injected Bone Marrow Cells to Newborn Mice Injected with an Angiogenesis Inhibitor
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Cho SJ, Ju SY, Woo SY, Kang HJ, Ahn HS, Ryu KH, Park EA
- KMID: 2099121
- J Korean Soc Neonatol.
- 2008 May;15(1):22-31.
PURPOSE: Bronchopulmonary dysplasia (BPD) is characterized by arrested vascular and alveolar growth in the premature lung. Considering the consequences of arrested lung growth, the idea of administering bone marrow cells... -
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- In vivo alternative testing with zebrafish in ecotoxicology
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Seok SH, Baek MW, Lee HY, Kim DJ, Na YR, Noh KJ, Park SH, Lee HK, Lee BH, Park JH
- KMID: 1104909
- J Vet Sci.
- 2008 Dec;9(4):351-357.
- doi: 10.4142/jvs.2008.9.4.351
Although rodents have previously been used in ecotoxicological studies, they are expensive, time-consuming, and are limited by strict legal restrictions. The present study used a zebrafish (Danio rerio) model and... -
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- Nucleolar translocalization of GRA10 of Toxoplasma gondii transfectionally expressed in HeLa cells
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Ahn HJ, Kim S, Nam HW
- KMID: 1104526
- Korean J Parasitol.
- 2007 Sep;45(3):165-174.
- doi: 10.3347/kjp.2007.45.3.165
Toxoplasma gondii GRA10 expressed as a GFP-GRA10 fusion protein in HeLa cells moved to the nucleoli within the nucleus rapidly and entirely. GRA10 was concentrated specifically in the dense fibrillar... -
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- Expression of Hepatitis C Virus Core Protein in Hepatocytes Does Not Modulate Proliferation or Apoptosis of CD8+ T Cells
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Jin YH, Crispe IN, Park S
- KMID: 2428300
- Yonsei Med J.
- 2005 Dec;46(6):827-834.
Hepatocytes are the primary targets of the hepatitis C virus (HCV). While immunosuppressive roles of HCV core protein have been found in several studies, it remains uncertain whether core protein... -
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- Establishment of an Orthotopic Mouse Non-Muscle Invasive Bladder Cancer Model Expressing the Mammalian Target of Rapamycin Signaling Pathway
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Kim SJ, Seo HK, Seo HH, Lee SJ, Kwon JK, Lee TJ, Chi BH, Chang IH
- KMID: 1734920
- J Korean Med Sci.
- 2014 Mar;29(3):343-350.
- doi: 10.3346/jkms.2014.29.3.343
We established an orthotopic non-muscle invasive bladder cancer (NMIBC) mouse model expressing the mammalian target of the rapamycin (mTOR) signaling pathway. After intravesical instillation of KU-7-lucs (day 0), animals were... -
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- Transcriptional targeting of gene expression in breast cancer by the promoters of protein regulator of cytokinesis 1 and ribonuclease reductase 2
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Yun HJ, Cho YH, Moon Y, Park YW, Yoon HK, Kim YJ, Cho SH, Lee YI, Kang BS, Kim WJ, Park K, Seo W
- KMID: 756337
- Exp Mol Med.
- 2008 Jun;40(3):345-353.
- doi: 10.3858/emm.2008.40.3.345
For cancer gene therapy, cancer-specific over-expression of a therapeutic gene is required to reduce side effects derived from expression of the gene in normal cells. To develop such an expression... -
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- Gene transfer using liposome-complexed adenovirus seems to overcome limitations due to coxsackievirus and adenovirus receptor-deficiency of cancer cells, both in vitro and in vivo
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Han SY, Lee YJ, Jung HI, Lee SW, Lim SJ, Hong SH, Jeong JS
- KMID: 1094122
- Exp Mol Med.
- 2008 Aug;40(4):427-434.
- doi: 10.3858/emm.2008.40.4.427
Use of adenoviruses as vehicle for gene therapy requires that target cells express appropriate receptors such as coxsakievirus and adenovirus receptor (CAR). We show here that CAR-deficiency in cancer cells,... -
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- Efficient gene delivery in differentiated human embryonic stem cells
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Kim JH, Do HJ, Choi SJ, Cho HJ, Park KH, Yang HM, Lee SH, Kim DK, Kwack K, Oh SK, Moon SY, Cha KY, Chung HM
- KMID: 1033099
- Exp Mol Med.
- 2005 Feb;37(1):36-44.
Human embryonic stem (hES) cells are capable of differentiating into pluralistic cell types, however, spontaneous differentiation generally gives rise to a limited number of specific differentiated cell types and a... -
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