J Vet Sci.  2007 Mar;8(1):15-20. 10.4142/jvs.2007.8.1.15.

Organotypic slice culture of the hypothalamic paraventricular nucleus of rat

Affiliations
  • 1Laboratory of Veterinary Pharmacology, BK21 Program for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. pandryu@plaza.snu.ac.kr
  • 2Department of Physiology, Institute of Health Science and Medical Research Center for Neural Dysfunction, Gyeongsang National University College of Medicine, Jinju 660-701, Korea.

Abstract

Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus (PVN) of rat. The results showed that the slice cultures maintain the typical shape of the nucleus, the immunocytochemical signals for oxytocin, vasopressin, and corticotropin-releasing hormone, and the electrophysiological properties of PVN neurons for up to 3 weeks in vitro. The PVN neurons in the culture expressed the green fluorescent protein gene that had been delivered by the adenoviral vectors. The results indicate that the cultured slices preserve the properties of the PVN neurons, and can be used in longterm studies on these neurons in vitro.

Keyword

electrophysiology; green fluorescent protein; immunocytochemistry; organotypic slice culture; paraventricular nucleus

MeSH Terms

Adenoviridae
Animals
Cell Culture Techniques/*methods
Corticotropin-Releasing Hormone/metabolism
Electrophysiology
Genetic Vectors
Green Fluorescent Proteins/metabolism
Immunohistochemistry
Neurons/*cytology/metabolism
Oxazines
Oxytocin/metabolism
Paraventricular Hypothalamic Nucleus/*anatomy & histology/cytology/metabolism
Rats
Vasopressins/metabolism
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