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Comparison of Serum-Free Media in RBC Differentiation from Human Hematopoietic Stem Cells

Kim JY, Kim S, Jeon YL, Choi Y, Kim HO

BACKGROUND: Research on RBC production from hematopoietic stem cells has been conducted competitively in many countries. However those were in vitro successes and many hurdles still remain for large scale...
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Suspension culture of Vero cells for the production of adenovirus type 5

Lee DK, Park J, Seo DW

PURPOSE: Most cell culture processes for viral vaccine production are mainly based on adherent cell culture systems using serum, which are associated with expensive and labor-intensive processes to produce large...
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Effect of Nitric Oxide on the Survival of R28 Cells

Lee JI, Kim JW

PURPOSE: To evaluate the effect of nitric oxide (NO) on the survival of R28 cells. METHODS: After immunostaining for GFAP, vimentin, S-100, and neurofilament, R28 cells were exposed to S-nitroso-N-acetyl-D, L-penicillamine...
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Effect of Creatine on the Survival of RGC-5 Cells under Serum Deprivation

Kim JW, Hong JH, Kang SH, Kim YY

PURPOSE: To evaluate the protective effect of creatine on the survival of retinal ganglion cells after serum deprivation. METHODS: RGC-5 cells were exposed to 5 mM creatine with serum-free media for...
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Establishment of Normal Human Middle Ear Epithelial (NHMEE) Cell Using Serum-free Media

Moon SK, Lee HK, Yoon JH, Chung MH, Kim HN

  • KMID: 2274491
  • Korean J Otolaryngol-Head Neck Surg.
  • 1998 Nov;41(11):1365-1371.
BACKGROUND AND OBJECTIVES: Despite many reports about middle ear mucosal epithelial cell culture of experimental animals like rat, gerbil or chinchilla, normal human middle ear epithelial (NHMEE) cells have not...
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Expression of cytokeratins and involucrin in cultured human keratinocytes

Song IH, Doh YH, Kim JY, Lee YC, Sung EG

  • KMID: 1937878
  • Korean J Anat.
  • 1998 Oct;31(5):663-671.
To evaluate the maturation and differentiation state of cultured keratinocytes, the author investigated expression of differentiation markers in cultured keratinocytes. The specimens were divided into three experimental groups, 3rd passage...
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Production and Quality Control of Adenoviral Vectors for Clinical Trials

Park SW, Sohn YS, Paik SY

The importance of recombinant adenoviral vectors for the development of gene therapy and prophylactic and therapeutic vaccines has led to efforts for process development of large scale production of clinically...
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Proliferative Effect of Retinal Glial Cells by Growth Factors

Cho NC, Lee MC, Han HJ, Ahn BG

  • KMID: 1952487
  • J Korean Ophthalmol Soc.
  • 1997 Jul;38(7):1179-1187.
The authors tried to evaluate the effect of the growth factors on cell proliferation in the human retina astrocyte culture. Each growth factor, EGF, bFGF, GGF, IGF-1 or PDGF, together...
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An experimental study on the stimulatory effects of epidermal growth factor and transforming growth factor-alpha on the growth of squamous cancer cell lines

Park YW

  • KMID: 2184118
  • J Korean Assoc Maxillofac Plast Reconstr Surg.
  • 1998 Nov;20(4):334-340.
Stimulatory effects of epidermal growth factor(EGF) and transforming growth factor-alpha(TGF-alpha) on the growth of squamous cancer cell lines established from human oral cancer tissue with moderate differentiation were studied in...
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The Role of Insulin-like Growth Factor I(IGF-I), and IGF Binding Protein (IGFBP) in Mouse Lung Cancer Cells

Cho CH, Kim SK, Kwak SM, Chang J, Kim SK, Chung KY

BACKGROUND: IGF-I is an important mitogen in many types of malignancies. Tumors also express many IGF binding proteins, which modulate IGF action. The purpose of this study was to evaluaste...
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Elevated Contractile Responses to Acetylcholine in Organ Cultured Rabbit Carotid Artery

Lee Y, Jung S, Won JE, Nam T, Ahn D

The aim of the present study was to examine the functional changes that occur when a rabbit carotid artery is cultured in serum-free medium. In endothelium (EC)-intact arteries cultured under...
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Long-Term Culture of Organotypic Hippocampal Slice from Old 3xTg-AD Mouse: An ex vivo Model of Alzheimer's Disease

Jang S, Kim H, Kim HJ, Lee SK, Kim EW, Namkoong K, Kim E

OBJECTIVE: Conventional methods for organotypic hippocampal tissue slice culture (OHSC) have shown several disadvantages or limitations regarding age of animals used, duration of culture and difficulty using neurodegenerative models. Therefore,...
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The effect of sodium fluoride and sodium orthovanadate on osteoblastic cell line MC3T3-E1 cells

Kim WJ, Chung KR

  • KMID: 2271229
  • Korean J Orthod.
  • 1991 Mar;21(1):97-111.
It is the aim of this study to investigate the effects of sodium fluoride and sodium orthovanadate upon the proliferation and activity of the osteoblast (MC3T3-E1 cells). MC3T3-E1 cells were...
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Effect of Intravenous Immuoglobulin on Interleukin-6 Production in Human Skeletal Muscle Cell Line (HM4)

Joo IS, Joo ES, Kim SH, Lee YB

  • KMID: 1956953
  • J Korean Neurol Assoc.
  • 2004 Jun;22(3):249-254.
BACKGROUND: Intravenous immunoglobulin (IVIg) has been widely used in the management of patients with various autoimmune neurological diseases, however, its action mechanisms have not fully been elucidated yet. This study...
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The Effect of the Photo-immobilized Epidermal Growth Factor on the Proliferation of the Anterior Cruciate Ligament Cell of the Knee

Kwon SY, Woo YK, Lee HS, Chung JW, Lee WH, Park YS

  • KMID: 2104897
  • J Korean Orthop Res Soc.
  • 2002 Oct;5(2):100-109.
PURPOSE: The purpose of this study is to find out the possibilities of the early treatment via artificial juxtacrine stimulation by photo-immobilization of growth factor in the anterior cruciate ligament...
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A Study in the Effects of Epidermal Growth Factor and Transforming Growth Factors-alpha on the Growth of Human Transitional Cell Carcinoma Cell Lines

Shin KY, Kim SJ, Choi WH, Choi DY, Park HY, Lee TY, Kong G, Woo YN, Kim DH

  • KMID: 2290025
  • Korean J Urol.
  • 1998 Jan;39(1):45-50.
PURPOSE: We evaluated the effects of epidermal growth factor(EGF) and transforming growth factor-alpha (TGF-alpha) on the growth of human transitional cell carcinoma cell lines. MATERIALS AND METHODS: T-24, KK47...
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Effects of Insulin-like Growth Factor in Peritoneal Fluid of Patients with Endometriosis on the Proliferation of Endometrial Stromal Cells

Kim JG, Suh CS, Kim SH, Choi YM, Moon SY, Lee JY

  • KMID: 1891279
  • Korean J Fertil Steril.
  • 1999 Dec;26(3):331-338.
The purposes of this study were to evaluate the effects of insulin-like growth factor (IGF)s in peritoneal fluid (PF) from patients with and without endometriosis on the proliferation of endometrial...
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The Effect of Interleukin-1 on The Production of Insulin-Like Growth Factors and Their Binding Proteins in Human Granulosa-Luteal Cells

Lee JY, Min EG, Kim JK, Suh CS, Kim SH, Choi YM, Moon SY

  • KMID: 2261050
  • Korean J Obstet Gynecol.
  • 1998 Jul;41(7):1795-1804.
The relationship between the immune system and the endocrine system in the ovary has become of an challenging and exciting subject to reproductive scientists. The intraovarian regulators such as insulin-like...
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Efficacy of Human Platelet Rich Fibrin Exudate vs Fetal Bovine Serum on Proliferation and Differentiation of Dental Pulp Stem Cells

Saeed MA, El-Rahman , Helal ME, Zaher AR, Grawish ME

BACKGROUND AND OBJECTIVES: The imperative role of dental pulp stem cells (DPSCs) in regenerative therapy demands an in-vitro expansion which must deal with the safety and ethical problems associated with...
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The effect of Insulin like growth factor-I (IGF-I) and IGF binding protein-3 (IGFBP-3) on cellular proliferation in mouse 3T3 fibroblast cells

Cho CH, Kwak SM, Moon TH, Cho JH, Ryu JS, Lee HL

  • KMID: 2318244
  • Tuberc Respir Dis.
  • 1999 Nov;47(5):618-628.
BACKGROUND: Cell growth is a balance between cell proliferation and cell death. Insulin-like growth factor-I(IGF-I), which binds IGF-I receptor (IGF-IR), mediates cellular proliferation as a potent mitogen. IGF binding protein-3...
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