Tuberc Respir Dis.  2001 May;50(5):549-556. 10.4046/trd.2001.50.5.549.

The Role of Insulin-like Growth Factor I(IGF-I), and IGF Binding Protein (IGFBP) in Mouse Lung Cancer Cells

• Cho CH
• Kim SK
• Kwak SM
• Chang J
• Kim SK
• Chung KY

• KMID: 2160197
• DOI: http://doi.org/10.4046/trd.2001.50.5.549

Abstract

BACKGROUND
IGF-I is an important mitogen in many types of malignancies. Tumors also express many IGF binding proteins, which modulate IGF action. The purpose of this study was to evaluaste the effect of IGF-I and IGFBP on cell proliferation in mouse lung cancer cells (3LL).
METHODS
The cellular proliferation of 3LL with the treatment of growth factors was evaluated using MTT assay. Western ligand blot was performed in order to determine whether 3LL cells secrete IGFBPs and we evaluated the effect of IGFBP on cellular proliferation.
RESULTS
The treatment of 3LL cells with IGF-I increased cellular proliferation in a serum free media. Western ligand blot of conditioned medium of 3LL with 125I-IGF-I demonstrated one single major band with an estimated molecular mass of 24 kDa. This band was identified as IGFBP-4 with immunoblot analysis using antisera. The addition of anti-IGFBP-4 antibody to abrogate the effect of IGFBP-4 resulted in increased cellular prolife ration suggesting that IGFBP-4 inhibits cell growth.
CONCLUSION
IGF-I increases cellular proliferation, however the secreted IGFBP- 4 has an ingibitory function on cell growth in 3LL. These findings suggest that IGF-I and IGFBP are involved in the cell proliferation.

Keyword

Insulin-like growth factor-I(IGF-I); IGF-binding protein (IGFBP); Cell proliferation

MeSH Terms

Animals
Carrier Proteins*
Cell Proliferation
Culture Media, Conditioned
Culture Media, Serum-Free
Immune Sera
Insulin-Like Growth Factor Binding Protein 4
Insulin-Like Growth Factor Binding Proteins
Insulin-Like Growth Factor I
Intercellular Signaling Peptides and Proteins
Lung Neoplasms*
Lung*
Mice*
Carrier Proteins
Culture Media, Conditioned
Culture Media, Serum-Free
Immune Sera
Insulin-Like Growth Factor Binding Protein 4
Insulin-Like Growth Factor Binding Proteins
Insulin-Like Growth Factor I
Intercellular Signaling Peptides and Proteins