J Korean Assoc Maxillofac Plast Reconstr Surg.  1998 Nov;20(4):334-340.

An experimental study on the stimulatory effects of epidermal growth factor and transforming growth factor-alpha on the growth of squamous cancer cell lines

• Park YW

Abstract

Stimulatory effects of epidermal growth factor(EGF) and transforming growth factor-alpha(TGF-alpha) on the growth of squamous cancer cell lines established from human oral cancer tissue with moderate differentiation were studied in vitro. After culturing in serum-free media for 24 hours, growth factors-EGF only, TGF-alpha only and EGF, TGF-alpha together- were added to the media and numbers of cells were analysed by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide(MTT) assay and compared with the control at 96, 144 hours. Each of EGF and TGF-alpha showed statistically significant stimulatory effects on the growth of cells respectively. Dose-dependent relationship of the stimulatory effects were not clearly demonstrated. The effects of EGF were higher than those of TGF-alpha and combinative administration showed higher effects than those of single uses. In conclusion, EGF may play an important and major role in differentiation and growth of human oral squamous cancer cells. TGF-alpha, produced from cells activated by EGF, also can stimulate the cell growth and could be an alternative ligand for EGF receptor.

Keyword

Epidermal growth factor; Transforming growth factor-alpha; Stimulatory effects

MeSH Terms

Cell Line*
Culture Media, Serum-Free
Epidermal Growth Factor*
Humans
Mouth Neoplasms
Receptor, Epidermal Growth Factor
Transforming Growth Factor alpha
Culture Media, Serum-Free
Epidermal Growth Factor
Receptor, Epidermal Growth Factor
Transforming Growth Factor alpha