Korean J Urol.
1998 Jan;39(1):45-50.
A Study in the Effects of Epidermal Growth Factor and Transforming Growth Factors-alpha on the Growth of Human Transitional Cell Carcinoma Cell Lines
- Affiliations
-
- 1Department of Urology, School of Medicine, Han Yang University, Seoul, Korea.
- 2Department of Pathology, School of Medicine, Han Yang University, Seoul, Korea.
- 3Department of Urology, National Police Hospital, Seoul, Korea.
Abstract
- PURPOSE: We evaluated the effects of epidermal growth factor(EGF) and transforming growth factor-alpha (TGF-alpha) on the growth of human transitional cell carcinoma cell lines.
MATERIALS AND METHODS
T-24, KK47 and KU-1 cell lines established from human transitional cell carcinoma tissues were used. We cultured the cell lines in serum-free media for 48 hours and then administered EGF and TGF-alpha in the concentrations of 0.1ng/ml, 1ng/ml, 10ng/ml and 100ng/m1 respectively. We analysed the effects by MTT assay at 144 hours.
RESULTS
There were statistically significant stimulatory effects on the growth of three cell lines in all cultures. Enhanced growth was also observed in the culture with administration of EGF and TGF-alpha simultaneously. None of stimulatory effects were dose-related.
CONCLUSIONS
We should take into consideration of possible role of EGF which are excreted in urine in very high concentration in a biologically active form and TGF-alpha of which urinary level increases with various type of bladder injuries in occurrence, progression and recurrence of transitional cell carcinoma of urothelium.
Keyword
MeSH Terms
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- An experimental study on the stimulatory effects of epidermal growth factor and transforming growth factor-alpha on the growth of squamous cancer cell lines
- Effect of epidermal growth factor on growth of human renal cell carcinoma and transitional cell carcinoma cell lines
- Role of Transforming Growth Factor Alpha in Hepatocarcinogenesis
- Expression of Epidermal Growth Factor Related Peptides, EGF-R, and c-erbB-2 and Their Relationship with the Prognostic Factors in Gastric Carcinoma
- Inhibition of Cell Growth and Suppression of c-myc Gene Expression by Transforming Growth Factor-beta1 in Cervical Carcinoma Cell Lines
