Korean J Orthod.  1991 Mar;21(1):97-111.

The effect of sodium fluoride and sodium orthovanadate on osteoblastic cell line MC3T3-E1 cells

• Kim WJ
• Chung KR

Abstract

It is the aim of this study to investigate the effects of sodium fluoride and sodium orthovanadate upon the proliferation and activity of the osteoblast (MC3T3-E1 cells). MC3T3-E1 cells were cultured in alpha-MEM containing 10% FBS and various concentration of sodium fluoride and sodium orthovanadate was appended to serum free media. DNA synthesis was examined through the [3H] thymidine incorporation into DNA. Collagen synthesis was examined through the [3H] proline incorporation into collagenase digestible protein and noncollagen protein. The following results were drawn; 1. Sodium fluoride stimulated the DNA synthesis of osteoblast significantly in dose-dependent manner within the concentration from 2micrometer to 10micrometer (P<0.005). 2. Sodium orthovanadate stimulated the DNA synthesis of osteoblast significantly in dosedependent manner within the concentration from 2micrometer to 8micrometer, however showed diminution at 10micrometer (P<0.001). 3. Sodium fluoride and sodium orthovanadate stimulated the percent collagen synthesis of osteoblast significantly in dose-dependent manner within the concentration from 5micrometer to 10micrometer (P<0.001). 4. Sodium fluoride and sodium orthovanadate stimulated the non collagen synthesis of osteoblast significantly in dose-dependent manner within the concentration from 5micrometer to 10micrometer (P<0.001). In conclusion, sodium fluoride and sodium orthovanadate stimulate the proliferation and activity of osteoblast by stimulation of DNA synthesis and collagen and noncollagen synthesis in osteoblast.

MeSH Terms

Cell Line*
Collagen
Collagenases
Culture Media, Serum-Free
DNA
Osteoblasts*
Proline
Sodium Fluoride*
Sodium*
Thymidine
Vanadates*
Collagen
Collagenases
Culture Media, Serum-Free
DNA
Proline
Sodium
Sodium Fluoride
Thymidine
Vanadates