Int J Oral Biol.  2014 Jun;39(2):121-128.

Effect of Sambucus sieboldiana Extract on the Cell Growth and Extracellular Matrix Formation in Osteoblast Cells

• Kim J
• Cho SH
• Park JT
• Yu SK
• Kim SG
• Kim DK

Abstract

Sambucus sieboldiana (SS) is a member of the family Caprifoliaceae and has been recommended as a functional material because of its several bioactivities. Although numerous literatures are available on the pharmacological and biological activities, the biological activity of SS in bone regeneration process has not yet been well-defined. Therefore, in this study, the effect of SS was investigated in the proliferation and differentiation of MC3T3-E1 osteoblastic cell line. The treatment of SS did not significantly affect the cell proliferation in MC3T3-E1 cells. SS significantly accelerated the mineralization and significantly increased the expression of alkaline phosphatase (ALP) and osteocalcin (OC) mRNAs, compared to the control, in the differentiation of MC3T3-E1 cells. SS significantly accelerated the decrease of osteonectin (ON) mRNA expression as compared with the control in a time-dependent manner in the differentiation of MC3T3-E1 cells. These results suggest that the SS facilitate the osteoblast differentiation and mineralization in MC3T3-E1 osteoblastic cells. Therefore, there may be potential properties for development and clinical application of bone regeneration materials.

Keyword

bone formation; Sambucus sieboldiana; differentiation; mineralization; osteoblasts

MeSH Terms

Alkaline Phosphatase
Bone Regeneration
Caprifoliaceae
Cell Line
Cell Proliferation
Extracellular Matrix*
Humans
Osteoblasts*
Osteocalcin
Osteogenesis
Osteonectin
RNA, Messenger
Sambucus*
Alkaline Phosphatase
Osteocalcin
Osteonectin
RNA, Messenger